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Two small <jats:styled-content style=\"fixed-case\">RNAs<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\"><jats:italic>B<\/jats:italic><\/jats:styled-content><jats:italic>rucella abortus<\/jats:italic>, Abc<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>1 and Abc<jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>2, are required for wild\u2010type virulence. Examination of the <jats:italic>abc<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content> loci revealed the presence of a gene encoding a <jats:styled-content style=\"fixed-case\">LysR<\/jats:styled-content>\u2010type transcriptional regulator flanking <jats:italic>abc<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content><jats:italic>2<\/jats:italic> on chromosome 1. Deletion of this <jats:italic>lys<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content> gene (<jats:italic>bab1_1517<\/jats:italic>) resulted in the complete loss of <jats:italic>abcR2<\/jats:italic> expression while no difference in <jats:italic>abc<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content><jats:italic>1<\/jats:italic> expression was observed. The <jats:styled-content style=\"fixed-case\"><jats:italic>B<\/jats:italic><\/jats:styled-content><jats:italic>. abortus bab1_1517<\/jats:italic> mutant strain was significantly attenuated in macrophages and mice, and <jats:italic>bab1_1517<\/jats:italic> was subsequently named <jats:italic>vtl<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content> for <jats:bold><jats:styled-content>v<\/jats:styled-content><\/jats:bold>irulence\u2010associated <jats:bold><jats:styled-content>t<\/jats:styled-content><\/jats:bold>ranscriptional <jats:styled-content style=\"fixed-case\"><jats:bold><jats:styled-content>L<\/jats:styled-content><\/jats:bold>ysR<\/jats:styled-content>\u2010family <jats:bold><jats:styled-content>r<\/jats:styled-content><\/jats:bold>egulator. Microarray analysis revealed three additional genes encoding small hypothetical proteins also under the control of <jats:styled-content style=\"fixed-case\">VtlR<\/jats:styled-content>. Electrophoretic mobility shift assays demonstrated that <jats:styled-content style=\"fixed-case\">VtlR<\/jats:styled-content> binds directly to the promoter regions of <jats:italic>abc<\/jats:italic><jats:styled-content style=\"fixed-case\"><jats:italic>R<\/jats:italic><\/jats:styled-content><jats:italic>2<\/jats:italic> and the three hypothetical protein\u2010encoding genes, and DNase <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> footprint analysis identified the specific nucleotide sequence in these promoters that <jats:styled-content style=\"fixed-case\">VtlR<\/jats:styled-content> binds to and drives gene expression. Strikingly, orthologs of <jats:styled-content style=\"fixed-case\">VtlR<\/jats:styled-content> are encoded in a wide range of host\u2010associated \u03b1\u2010proteobacteria, and it is likely that the <jats:styled-content style=\"fixed-case\">VtlR<\/jats:styled-content> genetic system represents a common regulatory circuit critical for host\u2013bacterium interactions.<\/jats:p>","DOI":"10.1111\/mmi.13123","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T09:25:02Z","timestamp":1436952302000},"page":"318-328","source":"Crossref","is-referenced-by-count":39,"title":["A <scp>LysR<\/scp>\u2010family transcriptional regulator required for virulence in <scp><i>B<\/i><\/scp><i>rucella abortus<\/i> is highly conserved among the \u03b1\u2010proteobacteria"],"prefix":"10.1111","volume":"98","author":[{"given":"Lauren M.","family":"Sheehan","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences and Pathobiology Center for Molecular 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