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Environmental stresses, such as starvation and antibiotics, can cause stalling of bacterial ribosomes, which may alter gene expression through a transcription or translation attenuation mechanism. In P<\/jats:italic><\/jats:styled-content>seudomonas aeruginosa<\/jats:italic>, the expression of MexXY<\/jats:styled-content> multidrug efflux system, which plays a significant role in resistance against aminoglycoside antibiotics, is controlled by a translation surveillance mechanism. Stalling of ribosome at the PA<\/jats:styled-content>5471 leader peptide (PA<\/jats:styled-content>5471.1) mRNA<\/jats:styled-content> leads to transcription of PA<\/jats:styled-content>5471, which subsequently up\u2010regulates the expression of MexXY<\/jats:styled-content>. In this study, we found that mutation in a suh<\/jats:italic>B<\/jats:italic><\/jats:styled-content> gene leads to decreased susceptibility to aminoglycosides. Transcriptomic analysis revealed an up\u2010regulation of MexXY<\/jats:styled-content> and PA<\/jats:styled-content>5471, which were demonstrated to be responsible for the decreased susceptibility of the suh<\/jats:italic>B<\/jats:italic><\/jats:styled-content> mutant. We further demonstrated that PA<\/jats:styled-content>5471.1 is essential for the up\u2010regulation of PA<\/jats:styled-content>5471 in the suh<\/jats:italic>B<\/jats:italic><\/jats:styled-content> mutant. Co\u2010immunoprecipitation assay revealed an interaction between SuhB<\/jats:styled-content> and ribosome, suggesting a role of SuhB<\/jats:styled-content> in translation. Indeed, higher amount of PA<\/jats:styled-content>5471.1 mRNA<\/jats:styled-content> was found to associate with ribosome isolated from the suh<\/jats:italic>B<\/jats:italic><\/jats:styled-content> mutant, indicating increased ribosome stalling. Therefore, this study identified SuhB<\/jats:styled-content> as a novel ribosome associated protein that is involved in modulating ribosome activity.<\/jats:p>","DOI":"10.1111\/mmi.13126","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T20:39:56Z","timestamp":1436906396000},"page":"370-383","source":"Crossref","is-referenced-by-count":36,"title":["SuhB<\/scp> is a novel ribosome associated protein that regulates expression of MexXY<\/scp> by modulating ribosome stalling in P<\/i><\/scp>seudomonas aeruginosa<\/i>"],"prefix":"10.1111","volume":"98","author":[{"given":"Jing","family":"Shi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education Department of Microbiology College of Life Sciences Nankai University Tianjin 300071 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