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The waterborne agent of cholera, <jats:styled-content style=\"fixed-case\"><jats:italic>V<\/jats:italic><\/jats:styled-content><jats:italic>ibrio cholerae<\/jats:italic>, encounters phosphate limitation in both the aquatic environment and human intestinal tract. This bacterium can utilize extracellular <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> (e<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>) as a phosphate source, a phenotype dependent on secreted endo\u2010 and exonucleases. However, no transporter of nucleotides has been identified in <jats:styled-content style=\"fixed-case\"><jats:italic>V<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009cholerae<\/jats:italic>, suggesting that in order for the organism to utilize the <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> as a phosphate source, it must first separate the phosphate and nucleoside groups before transporting phosphate into the cell. In this study, we investigated the factors required for assimilation of phosphate from e<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. We identified <jats:styled-content style=\"fixed-case\">PhoX<\/jats:styled-content>, and the previously unknown proteins <jats:styled-content style=\"fixed-case\">UshA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CpdB<\/jats:styled-content> as the major phosphatases that allow phosphate acquisition from e<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and nucleotides. We demonstrated separable but partially overlapping roles for the three phosphatases and showed that the activity of <jats:styled-content style=\"fixed-case\">PhoX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CpdB<\/jats:styled-content> is induced by phosphate limitation. Thus, this study provides mechanistic insight into how <jats:styled-content style=\"fixed-case\"><jats:italic>V<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009cholerae<\/jats:italic> can acquire phosphate from extracellular <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>, which is likely to be an important phosphate source in the environment and during infection.<\/jats:p>","DOI":"10.1111\/mmi.13128","type":"journal-article","created":{"date-parts":[[2015,7,15]],"date-time":"2015-07-15T09:25:14Z","timestamp":1436952314000},"page":"453-469","source":"Crossref","is-referenced-by-count":40,"title":["<scp><i>V<\/i><\/scp><i>ibrio cholerae<\/i> phosphatases required for the utilization of nucleotides and extracellular <scp>DNA<\/scp> as phosphate sources"],"prefix":"10.1111","volume":"99","author":[{"given":"EmilyKate","family":"McDonough","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology Tufts University School of Medicine  Boston MA 02111 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Kamp","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology Tufts University School of Medicine  Boston MA 02111 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Camilli","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology Tufts University School of Medicine  Boston MA 02111 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04945.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1016\/S0021-9258(18)97738-0","article-title":"A new cyclic phosphodiesterase having a 3\u2032\u2010nucleotidase activity from Escherichia coli b. 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