{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:15:13Z","timestamp":1791400513160,"version":"4.3.3"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T00:00:00Z","timestamp":1440979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["AI 084734"],"award-info":[{"award-number":["AI 084734"]}]},{"name":"AI","award":["048417"],"award-info":[{"award-number":["048417"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"Summary<\/jats:title>The regulators of M<\/jats:italic><\/jats:styled-content>ycobacterium tuberculosis<\/jats:italic>\u2005DNA<\/jats:styled-content> replication are largely unknown. Here, we demonstrate that in synchronously replicating M<\/jats:italic><\/jats:styled-content>. tuberculosis<\/jats:italic>, MtrA<\/jats:styled-content> access to origin of replication (ori<\/jats:italic>C<\/jats:styled-content><\/jats:italic>) is enriched in the post\u2010replication (D<\/jats:styled-content>) period. The increased ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> binding results from elevated MtrA<\/jats:styled-content> phosphorylation (MtrA<\/jats:styled-content>\u223cP<\/jats:styled-content>) as evidenced by reduced expression of dna<\/jats:italic>N<\/jats:italic><\/jats:styled-content>, dna<\/jats:italic>A<\/jats:italic><\/jats:styled-content> and increased expression of select cell division targets. Overproduction of gain\u2010of\u2010function MtrAY102C<\/jats:sub><\/jats:styled-content> advanced the MtrA<\/jats:styled-content>\u2005ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> access to the C<\/jats:styled-content> period, reduced dna<\/jats:italic>A<\/jats:italic><\/jats:styled-content> and dna<\/jats:italic>N<\/jats:italic><\/jats:styled-content> expression, interfered with replication synchrony and compromised cell division. Overproduction of wild\u2010type (MtrA<\/jats:styled-content>+) or phosphorylation\u2010defective MtrAD56N<\/jats:sub><\/jats:styled-content> did not promote ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> access in the C<\/jats:styled-content> period, nor affected cell cycle progression. MtrA<\/jats:styled-content> interacts with DnaA<\/jats:styled-content> signaling a possibility that DnaA<\/jats:styled-content> helps load MtrA<\/jats:styled-content> on ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content>. Therefore, ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> sequestration by MtrA<\/jats:styled-content>\u223cP<\/jats:styled-content> in the D<\/jats:styled-content> period may normally serve to prevent untimely initiations and that DnaA<\/jats:styled-content>\u2013MtrA<\/jats:styled-content> interactions may facilitate regulated ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> replication. Finally, despite the near sequence identity of MtrA<\/jats:styled-content> in M<\/jats:italic><\/jats:styled-content>. smegmatis<\/jats:italic> and M<\/jats:italic><\/jats:styled-content>. tuberculosis<\/jats:italic>, the M<\/jats:italic><\/jats:styled-content>. smegmatis ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> is not MtrA<\/jats:styled-content>\u2010target. We conclude that M<\/jats:italic><\/jats:styled-content>. tuberculosis ori<\/jats:italic>C<\/jats:italic><\/jats:styled-content> has evolved to be regulated by MtrA<\/jats:styled-content> and that cell cycle progression in this organisms are governed, at least in part, by oscillations in the MtrA<\/jats:styled-content>\u223cP<\/jats:styled-content> levels.<\/jats:p>","DOI":"10.1111\/mmi.13144","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T10:26:47Z","timestamp":1437733607000},"page":"586-604","source":"Crossref","is-referenced-by-count":41,"title":["M<\/i><\/scp>ycobacterium tuberculosis ori<\/i>C<\/scp> sequestration by MtrA<\/scp> response regulator"],"prefix":"10.1111","volume":"98","author":[{"given":"Gorla","family":"Purushotham","sequence":"first","affiliation":[{"name":"Biomedical Research The University of Health Science Center at Tyler Tyler TX 75708 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishna 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