{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:31:47Z","timestamp":1790735507898,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,8,22]],"date-time":"2015-08-22T00:00:00Z","timestamp":1440201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R37 GM55425"],"award-info":[{"award-number":["R37 GM55425"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University Grants Commission, Government of India"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"Summary<\/jats:title>\n \n Trivalent organoarsenic compounds are far more toxic than either pentavalent organoarsenicals or inorganic arsenite. Many microbes methylate inorganic arsenite (\n A<\/jats:styled-content>\n s(\n III<\/jats:styled-content>\n )) to more toxic and carcinogenic methylarsenite (\n MAs<\/jats:styled-content>\n (\n III<\/jats:styled-content>\n )). Additionally, monosodium methylarsenate (\n MSMA<\/jats:styled-content>\n or\n MAs<\/jats:styled-content>\n (\n V<\/jats:styled-content>\n )) has been used widely as an herbicide and is reduced by microbial communities to\n MAs<\/jats:styled-content>\n (\n III<\/jats:styled-content>\n ). Roxarsone (3\u2010nitro\u20104\u2010hydroxybenzenearsonic acid) is a pentavalent aromatic arsenical that is used as antimicrobial growth promoter for poultry and swine, and its active form is the trivalent species\n R<\/jats:styled-content>\n ox(\n III<\/jats:styled-content>\n ). A bacterial permease,\n ArsP<\/jats:styled-content>\n , from\n \n C<\/jats:italic>\n <\/jats:styled-content>\n ampylobacter jejuni<\/jats:italic>\n , was recently shown to confer resistance to roxarsone. In this study,\n \n C<\/jats:italic>\n <\/jats:styled-content>\n .\u2009jejuni<\/jats:italic>\n \u2005\n \n arsP<\/jats:italic>\n <\/jats:styled-content>\n was expressed in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n scherichia coli<\/jats:italic>\n and shown to confer resistance to\n MAs<\/jats:styled-content>\n (\n III<\/jats:styled-content>\n ) and\n R<\/jats:styled-content>\n ox(\n III<\/jats:styled-content>\n ) but not to inorganic\n A<\/jats:styled-content>\n s(\n III<\/jats:styled-content>\n ) or pentavalent organoarsenicals. Cells of\n \n E<\/jats:italic>\n <\/jats:styled-content>\n .\u2009coli<\/jats:italic>\n expressing\n \n arsP<\/jats:italic>\n <\/jats:styled-content>\n did not accumulate trivalent organoarsenicals. Everted membrane vesicles from those cells accumulated\n MAs<\/jats:styled-content>\n (\n III<\/jats:styled-content>\n )\u2009>\u2009\n R<\/jats:styled-content>\n ox(\n III<\/jats:styled-content>\n ) with energy supplied by\n NADH<\/jats:styled-content>\n oxidation, reflecting efflux from cells. The vesicles did not transport\n A<\/jats:styled-content>\n s(\n III<\/jats:styled-content>\n ),\n MAs<\/jats:styled-content>\n (\n V<\/jats:styled-content>\n ) or pentavalent roxarsone. Mutation or modification of the two conserved cysteine residues resulted in loss of transport activity, suggesting that they play a role in\n ArsP<\/jats:styled-content>\n function. Thus,\n ArsP<\/jats:styled-content>\n is the first identified efflux system specific for trivalent organoarsenicals.\n <\/jats:p>","DOI":"10.1111\/mmi.13145","type":"journal-article","created":{"date-parts":[[2015,8,3]],"date-time":"2015-08-03T03:07:56Z","timestamp":1438571276000},"page":"625-635","source":"Crossref","is-referenced-by-count":111,"title":["ArsP<\/scp>\n : a methylarsenite efflux permease"],"prefix":"10.1111","volume":"98","author":[{"given":"Jian","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine Florida International University Miami FL 33199 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahendra","family":"Madegowda","sequence":"additional","affiliation":[{"name":"Department of Cellular Biology and Pharmacology, Herbert Wertheim College of Medicine Florida International University Miami FL 33199 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