{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:55:41Z","timestamp":1790362541067,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,11,17]],"date-time":"2015-11-17T00:00:00Z","timestamp":1447718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>higella sonnei<\/jats:italic> is a bacterial pathogen and causative agent of bacillary dysentery. It deploys a type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> secretion system to inject effector proteins into host epithelial cells and macrophages, an essential step for tissue invasion and immune evasion. Although the arsenal of bacterial effectors and their cellular targets have been studied extensively, little is known about the prerequisites for deployment of type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> secreted proteins during infection. Here, we describe a novel <jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009sonnei<\/jats:italic> adhesin, SSO1327 which is a multivalent adhesion molecule (<jats:styled-content style=\"fixed-case\">MAM<\/jats:styled-content>) required for invasion of epithelial cells and macrophages and for infection <jats:italic>in vivo<\/jats:italic>. The <jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009sonnei<\/jats:italic>\u2005<jats:styled-content style=\"fixed-case\">MAM<\/jats:styled-content> mediates intimate attachment to host cells, which is required for efficient translocation of type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> effectors into host cells. SSO1327 is non\u2010redundant to <jats:styled-content style=\"fixed-case\">IcsA<\/jats:styled-content>; its activity is independent of type <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> secretion. In contrast to the up\u2010regulation of <jats:styled-content style=\"fixed-case\">IcsA<\/jats:styled-content>\u2010dependent and independent attachment and invasion by deoxycholate in <jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>higella flexneri<\/jats:italic>, deoxycholate negatively regulates <jats:styled-content style=\"fixed-case\">IcsA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MAM<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\"><jats:italic>S<\/jats:italic><\/jats:styled-content><jats:italic>.\u2009sonnei<\/jats:italic> resulting in reduction in attachment and invasion and virulence attenuation <jats:italic>in vivo<\/jats:italic>. A strain deficient for SSO1327 is avirulent <jats:italic>in vivo<\/jats:italic>, but still elicits a host immune response.<\/jats:p>","DOI":"10.1111\/mmi.13255","type":"journal-article","created":{"date-parts":[[2015,10,20]],"date-time":"2015-10-20T11:18:13Z","timestamp":1445339893000},"page":"658-673","source":"Crossref","is-referenced-by-count":32,"title":["The Multivalent Adhesion Molecule SSO1327 plays a key role in <i>Shigella sonnei<\/i> pathogenesis"],"prefix":"10.1111","volume":"99","author":[{"given":"Rasha Y.","family":"Mahmoud","sequence":"first","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS) University of Strathclyde  Glasgow UK"},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy Helwan University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Henry","family":"Stones","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Infection, School of 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