{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:18:54Z","timestamp":1777940334750,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,11,27]],"date-time":"2015-11-27T00:00:00Z","timestamp":1448582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["HI 1511\/2-1"],"award-info":[{"award-number":["HI 1511\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"German Research Foundation","doi-asserted-by":"crossref","award":["HI 1511\/5-1"],"award-info":[{"award-number":["HI 1511\/5-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["31003A_153200"],"award-info":[{"award-number":["31003A_153200"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003762","name":"Institut Pasteur","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Institut Carnot-Pasteur MI"},{"name":"French Region Ile de France","award":["ANR-10-LABX-62-IBEID"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID"]}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale (FRM)","doi-asserted-by":"crossref","award":["DEQ20120323697"],"award-info":[{"award-number":["DEQ20120323697"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Pasteur-Weizmann"},{"name":"Knut and Alice Wallenberg"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,2]]},"abstract":"Summary<\/jats:title>The causative agent of Legionnaires' disease, L<\/jats:italic><\/jats:styled-content>egionella pneumophila<\/jats:italic>, employs the autoinducer compound LAI<\/jats:styled-content>\u20101 (3\u2010hydroxypentadecane\u20104\u2010one) for cell\u2013cell communication. LAI<\/jats:styled-content>\u20101 is produced and detected by the L<\/jats:styled-content>qs (L<\/jats:italic><\/jats:styled-content>egionella<\/jats:italic> quorum sensing) system, comprising the autoinducer synthase LqsA<\/jats:styled-content>, the sensor kinases LqsS<\/jats:styled-content> and LqsT<\/jats:styled-content>, as well as the response regulator LqsR<\/jats:styled-content>. L<\/jats:styled-content>qs\u2010regulated processes include pathogen\u2013host interactions, production of extracellular filaments and natural competence for DNA<\/jats:styled-content> uptake. Here we show that synthetic LAI<\/jats:styled-content>\u20101 promotes the motility of L<\/jats:italic><\/jats:styled-content>. pneumophila<\/jats:italic> by signalling through LqsS<\/jats:styled-content>\/LqsT<\/jats:styled-content> and LqsR<\/jats:styled-content>. Upon addition of LAI<\/jats:styled-content>\u20101, autophosphorylation of LqsS<\/jats:styled-content>\/LqsT<\/jats:styled-content> by [\u03b3\u201032<\/jats:sup>P<\/jats:styled-content>]\u2010ATP<\/jats:styled-content> was inhibited in a dose\u2010dependent manner. In contrast, the V<\/jats:italic><\/jats:styled-content>ibrio cholerae<\/jats:italic> autoinducer CAI<\/jats:styled-content>\u20101 (3\u2010hydroxytridecane\u20104\u2010one) promoted the phosphorylation of LqsS<\/jats:styled-content> (but not LqsT<\/jats:styled-content>). LAI<\/jats:styled-content>\u20101 did neither affect the stability of phospho\u2010LqsS<\/jats:styled-content> or phospho\u2010LqsT<\/jats:styled-content>, nor the dephosphorylation by LqsR<\/jats:styled-content>. Transcriptome analysis of L<\/jats:italic><\/jats:styled-content>. pneumophila<\/jats:italic> treated with LAI<\/jats:styled-content>\u20101 revealed that the compound positively regulates a number of genes, including the non\u2010coding RNAs<\/jats:styled-content>\u2005rsm<\/jats:italic>Y<\/jats:italic><\/jats:styled-content> and rsm<\/jats:italic>Z<\/jats:italic><\/jats:styled-content>, and negatively regulates the RNA<\/jats:styled-content>\u2010binding global regulator crs<\/jats:italic>A<\/jats:italic><\/jats:styled-content>. Accordingly, LAI<\/jats:styled-content>\u20101 controls the switch from the replicative to the transmissive growth phase of L<\/jats:italic><\/jats:styled-content>. pneumophila<\/jats:italic>. In summary, the findings indicate that LAI<\/jats:styled-content>\u20101 regulates motility and the biphasic life style of L<\/jats:italic><\/jats:styled-content>. pneumophila<\/jats:italic> through LqsS<\/jats:styled-content>\u2010 and LqsT<\/jats:styled-content>\u2010dependent phosphorylation signalling.<\/jats:p>","DOI":"10.1111\/mmi.13265","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T08:06:41Z","timestamp":1446710801000},"page":"778-793","source":"Crossref","is-referenced-by-count":46,"title":["The \u03b1\u2010hydroxyketone LAI\u20101 regulates motility, Lqs\u2010dependent phosphorylation signalling and gene expression of Legionella pneumophila<\/i>"],"prefix":"10.1111","volume":"99","author":[{"given":"Ursula","family":"Schell","sequence":"first","affiliation":[{"name":"Max von Pettenkofer Institute Ludwig\u2010Maximilians University Pettenkoferstra\u00dfe 9a 80336 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Simon","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute Ludwig\u2010Maximilians University Pettenkoferstra\u00dfe 9a 80336 Munich Germany"},{"name":"Institute of Medical Microbiology University of Z\u00fcrich Gloriastrasse 30\/32 8006 Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Sahr","sequence":"additional","affiliation":[{"name":"Institut Pasteur Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires Paris France"},{"name":"CNRS UMR 3525 28 Rue du Dr Roux 75724 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominik","family":"Hager","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ludwig\u2010Maximilians University Butenandtstrasse 5\u201013, Building F 81377 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael F.","family":"Albers","sequence":"additional","affiliation":[{"name":"Chemical Biology Center (KBC) Institute of Chemistry Ume\u00e5 University S\u201090187 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aline","family":"Kessler","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute Ludwig\u2010Maximilians University Pettenkoferstra\u00dfe 9a 80336 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Fahrnbauer","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ludwig\u2010Maximilians University Butenandtstrasse 5\u201013, Building F 81377 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Trauner","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ludwig\u2010Maximilians University Butenandtstrasse 5\u201013, Building F 81377 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Hedberg","sequence":"additional","affiliation":[{"name":"Chemical Biology Center (KBC) Institute of Chemistry Ume\u00e5 University S\u201090187 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Institut Pasteur Unit\u00e9 de Biologie des Bact\u00e9ries Intracellulaires Paris France"},{"name":"CNRS UMR 3525 28 Rue du Dr Roux 75724 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Hilbi","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer Institute Ludwig\u2010Maximilians University Pettenkoferstra\u00dfe 9a 80336 Munich Germany"},{"name":"Institute of Medical Microbiology University of Z\u00fcrich Gloriastrasse 30\/32 8006 Z\u00fcrich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01081-08"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.2.575-585.2001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02465.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00703.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3029-3034.1998"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094226"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099776"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911764107"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00060"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05243.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00141"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12450"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2003.12.003"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm907"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0882-4010(03)00027-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.16.4879-4889.1999"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01519.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02884.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.17-23.1997"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06284"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1758-2229.2011.00247.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12235"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100109-104034"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1967"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.9.2540-2547.2004"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.07.042"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.237"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02889.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00050-8"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.4.1527-1532.2005"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2885-2895.2006"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00829-2"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03706.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04129.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00052-09"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-102108-134304"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07548.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06705.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.13.1.115-119.1981"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1658-1666.2004"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06677.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.20270"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12612"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.01.003"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801929200"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/s120302899"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.01005.x"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01002-08"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2010.03.004"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02167.x"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020046"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb1003652"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.07992.x"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.12.004"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05604.x"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13265","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T18:20:06Z","timestamp":1696270806000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,27]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1111\/mmi.13265"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13265","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,27]]}}}