{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:06:26Z","timestamp":1792220786631,"version":"4.4.0"},"reference-count":82,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,22]],"date-time":"2015-12-22T00:00:00Z","timestamp":1450742400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institutes of General Medical Sciences","doi-asserted-by":"crossref","award":["GM061019"],"award-info":[{"award-number":["GM061019"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"Summary<\/jats:title>\n \n Bacterial morphology is determined primarily by the architecture of the peptidoglycan (\n PG<\/jats:styled-content>\n ) cell wall, a mesh\u2010like layer that encases the cell. To identify novel mechanisms that create or maintain cell shape in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n scherichia coli<\/jats:italic>\n , we used flow cytometry to screen a transposon insertion library and identified a\n wec<\/jats:italic>\n \n E<\/jats:italic>\n <\/jats:styled-content>\n mutant that altered cell shape, causing cells to filament and swell.\n WecE<\/jats:styled-content>\n is a sugar aminotransferase involved in the biosynthesis of enterobacterial common antigen (\n ECA<\/jats:styled-content>\n ), a non\u2010essential outer membrane glycolipid of the\n \n E<\/jats:italic>\n <\/jats:styled-content>\n nterobacteriaceae<\/jats:italic>\n . Loss of\n wec<\/jats:italic>\n \n E<\/jats:italic>\n <\/jats:styled-content>\n interrupts biosynthesis of\n ECA<\/jats:styled-content>\n and causes the accumulation of the undecaprenyl pyrophosphate\u2010linked intermediate\n ECA<\/jats:styled-content>\n \u2010lipid\n II<\/jats:styled-content>\n . The\n wec<\/jats:italic>\n \n E<\/jats:italic>\n <\/jats:styled-content>\n shape defects were reversed by: (i) preventing initiation of\n ECA<\/jats:styled-content>\n biosynthesis, (ii) increasing the synthesis of the lipid carrier undecaprenyl phosphate (\n U<\/jats:styled-content>\n nd\u2010\n P<\/jats:styled-content>\n ), (iii) diverting\n U<\/jats:styled-content>\n nd\u2010\n P<\/jats:styled-content>\n to\n PG<\/jats:styled-content>\n synthesis or (iv) promoting\n U<\/jats:styled-content>\n nd\u2010\n P<\/jats:styled-content>\n recycling. The results argue that the buildup of\n ECA<\/jats:styled-content>\n \u2010lipid\n II<\/jats:styled-content>\n sequesters part of the pool of\n U<\/jats:styled-content>\n nd\u2010\n P<\/jats:styled-content>\n , which, in turn, adversely affects\n PG<\/jats:styled-content>\n synthesis. The data strongly suggest there is competition for a common pool of\n U<\/jats:styled-content>\n nd\u2010\n P<\/jats:styled-content>\n , whose proper distribution to alternate metabolic pathways is required to maintain normal cell shape in\n \n E<\/jats:italic>\n <\/jats:styled-content>\n .\u2009coli<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/mmi.13284","type":"journal-article","created":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T01:34:44Z","timestamp":1448242484000},"page":"1-14","source":"Crossref","is-referenced-by-count":135,"title":["Dead\u2010end intermediates in the enterobacterial common antigen pathway induce morphological defects in\n Escherichia coli<\/i>\n by competing for undecaprenyl phosphate"],"prefix":"10.1111","volume":"100","author":[{"given":"Matthew A.","family":"Jorgenson","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University of Arkansas for Medical Sciences Little Rock AR 72205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh","family":"Kannan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Arkansas for Medical Sciences Little Rock AR 72205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary E.","family":"Laubacher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Arkansas for Medical Sciences Little Rock AR 72205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1760-281X","authenticated-orcid":false,"given":"Kevin D.","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Arkansas for Medical Sciences Little Rock AR 72205 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01244-09"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201490730"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03511.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04063.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.22.6688-6693.2001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12703"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1574-6976.2007.00090.x","article-title":"Morphogenesis of rod\u2010shaped sacculi","volume":"32","author":"Blaauwen T.","year":"2008","journal-title":"FEMS Microbiol Rev"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00089.x"},{"key":"e_1_2_6_10_1","first-page":"1159","article-title":"Thermosensitive Bacillus subtilis mutants which lyse at the nonpermissive temperature","volume":"133","author":"Brandt C.","year":"1987","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_11_1","first-page":"1325","article-title":"Mutants of Bacillus\u2010subtilis 168 thermosensitive for growth and wall teichoic\u2010acid synthesis","volume":"135","author":"Briehl M.","year":"1989","journal-title":"J Gen Microbiol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060964"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1128\/JB.181.3.973-980.1999","article-title":"Effect of wzx (rfbX) mutations on A\u2010band and B\u2010band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5","volume":"181","author":"Burrows L.L.","year":"1999","journal-title":"J Bacteriol"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.12.3784-3789.1993"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05938-11"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00839-10"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01348-07"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.22.5875-5884.1998"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01336-06"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00197-06"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.04.009"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06818.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00596-13"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1511751112"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.21.6.950"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.6.1913-1922.2005"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02499.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6093-6099.2002"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00022-11"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003663"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2004.04.015"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1128\/jb.136.3.1143-1158.1978","article-title":"Morphology of an Escherichia coli mutant with a temperature\u2010dependent round cell shape","volume":"136","author":"Iwaya M.","year":"1978","journal-title":"J Bacteriol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03504.x"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.9.2733-2738.1999"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01700.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01740-07"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01450-12"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-140-1-49"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnv053"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.59.050405.101230"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.19.5539-5546.1995"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00461-06"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.17.5748-5752.1992"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.24.8326-8336.2004"},{"key":"e_1_2_6_46_1","doi-asserted-by":"crossref","first-page":"13490","DOI":"10.1016\/S0021-9258(18)77373-0","article-title":"Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis","volume":"265","author":"Meier\u2010Dieter U.","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.61"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2814"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004056"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06052-11"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.4.1147-1152.2003"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-137-4-921"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08023.x"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00415-07"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.10.024"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.17.5328-5332.2003"},{"key":"e_1_2_6_57_1","doi-asserted-by":"crossref","first-page":"4008","DOI":"10.1128\/jb.170.9.4008-4014.1988","article-title":"Accumulation of a lipid\u2010linked intermediate involved in enterobacterial common antigen synthesis in Salmonella typhimurium mutants lacking dTDP\u2010glucose pyrophosphorylase","volume":"170","author":"Rick P.D.","year":"1988","journal-title":"J Bacteriol"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301750200"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1689"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1254522"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11367"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12058"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.13.4144-4156.1994"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12405"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/006312-0"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.10.027"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2677"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2015.01.011"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6768-6774.2004"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01812-08"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00455-07"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12473"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00094.x"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00099.x"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.043"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.2.508-520.1999"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142545"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01938-06"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209742109"},{"key":"e_1_2_6_81_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00001-06"},{"key":"e_1_2_6_82_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134102"},{"key":"e_1_2_6_83_1","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1128\/jb.100.1.433-444.1969","article-title":"Biosynthesis of cell wall lipopolysaccharide in mutants of Salmonella. V. A mutant of Salmonella typhimurium defective in the synthesis of cytidine diphosphoabequose","volume":"100","author":"Yuasa R.","year":"1969","journal-title":"J Bacteriol"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13284","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13284","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T04:00:55Z","timestamp":1696219255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13284"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,22]]},"references-count":82,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1111\/mmi.13284"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13284","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725956763.793512124","asserted-by":"object"}]},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,22]]}}}