{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:43:43Z","timestamp":1791899023832,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T00:00:00Z","timestamp":1454976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    is a human pathogen causing atypical pneumonia with a minimalized and highly streamlined genome. So far, hydrogen peroxide production, cytadherence, and the ADP\u2010ribosylating CARDS toxin have been identified as pathogenicity determinants. We have studied haemolysis caused by\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    , and discovered that hydrogen peroxide is responsible for the oxidation of heme, but not for lysis of erythrocytes. This feature could be attributed to hydrogen sulfide, a compound that has previously not been identified as virulence factor in lung pathogens. Indeed, we observed hydrogen sulfide production by\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    . The search for a hydrogen sulfide\u2010producing enzyme identified HapE, a protein with similarity to cysteine desulfurases. In contrast to typical cysteine desulfurases, HapE is a bifunctional enzyme: it has both the cysteine desulfurase activity to produce alanine and the cysteine desulfhydrase activity to produce pyruvate and hydrogen sulfide. Experiments with purified HapE showed that the enzymatic activity of the protein is responsible for haemolysis, demonstrating that HapE is a novel potential virulence factor of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mmi.13300","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T04:50:49Z","timestamp":1451364649000},"page":"42-54","source":"Crossref","is-referenced-by-count":51,"title":["Hydrogen sulfide is a novel potential virulence factor of\n                    <scp>\n                      <i>M<\/i>\n                    <\/scp>\n                    <i>ycoplasma pneumoniae<\/i>\n                    : characterization of the unusual cysteine desulfurase\/desulfhydrase HapE"],"prefix":"10.1111","volume":"100","author":[{"given":"Stephanie","family":"Gro\u00dfhennig","sequence":"first","affiliation":[{"name":"Department of General Microbiology Georg\u2010August\u2010University G\u00f6ttingen Grisebachstr. 8 D\u201037077 G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"Ischebeck","sequence":"additional","affiliation":[{"name":"Department of Plant Biochemistry Georg\u2010August\u2010University, Albrecht\u2010von\u2010Haller\u2010Institute Justus\u2010von\u2010Liebig Weg 11 D\u201037077 G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Gibhardt","sequence":"additional","affiliation":[{"name":"Department of General Microbiology Georg\u2010August\u2010University G\u00f6ttingen Grisebachstr. 8 D\u201037077 G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Busse","sequence":"additional","affiliation":[{"name":"Department of General Microbiology Georg\u2010August\u2010University G\u00f6ttingen Grisebachstr. 8 D\u201037077 G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivo","family":"Feussner","sequence":"additional","affiliation":[{"name":"Department of Plant Biochemistry Georg\u2010August\u2010University, Albrecht\u2010von\u2010Haller\u2010Institute Justus\u2010von\u2010Liebig Weg 11 D\u201037077 G\u00f6ttingen Germany"},{"name":"Georg\u2010August\u2010University, G\u00f6ttingen Center for Molecular Biosciences (GZMB) 37077 G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"St\u00fclke","sequence":"additional","affiliation":[{"name":"Department of General Microbiology Georg\u2010August\u2010University G\u00f6ttingen Grisebachstr. 8 D\u201037077 G\u00f6ttingen Germany"},{"name":"Georg\u2010August\u2010University, G\u00f6ttingen Center for Molecular Biosciences (GZMB) 37077 G\u00f6ttingen 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