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Macrolide antibiotic rapamycin, in complex with a cytosol protein named FKBP12, specifically inhibits TORC1, causing growth arrest. The FKBP12\u2010rapamycin complex interferes with TORC1 function by binding to the FRB domain of the TOR proteins. In an attempt to understand the role of the FRB domain in TOR function, we identified a single point mutation (Tor2<jats:sup>W2041R<\/jats:sup>) in the FRB domain of Tor2 that renders yeast cells rapamycin resistant and temperature sensitive. At the permissive temperature, the Tor2 mutant protein is partially defective for binding with Kog1 and TORC1 is impaired for membrane association. At the restrictive temperature, Kog1 but not the Tor2 mutant protein, is rapidly degraded. Overexpression of ubiquitin stabilizes Kog1 and suppresses the growth defect associated with the<jats:italic>tor2<\/jats:italic>mutant at the nonpremissive temperature. We find that ubiquitin binds non\u2010covalently to Kog1, prevents Kog1 from degradation and stabilizes TORC1. Our data reveal a unique role for ubiquitin in regulation of TORC1 and suggest that Kog1 requires association with the Tor proteins for stabilization.<\/jats:p>","DOI":"10.1111\/mmi.13319","type":"journal-article","created":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T00:10:57Z","timestamp":1450915857000},"page":"303-314","source":"Crossref","is-referenced-by-count":16,"title":["Ubiquitin regulates TORC1 in yeast<scp><i>S<\/i><\/scp><i>accharomyces cerevisiae<\/i>"],"prefix":"10.1111","volume":"100","author":[{"given":"Kejin","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh PA 15213 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuguang","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Biology Henan University Kaifeng 475000 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gonghong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh PA 15213 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Yuan","sequence":"additional","affiliation":[{"name":"School of Life Science and Biotechnology Dalian University of Technology Dalian 116024 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Medical and Healthcare Center, Hainan Provincial People's Hospital Haikou 570311 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine Pittsburgh PA 15213 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