{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T16:36:26Z","timestamp":1720629386143},"reference-count":79,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T00:00:00Z","timestamp":1457568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The bacterial phosphotransferase system (PTS) transports and phosphorylates sugars, but also carries out numerous regulatory functions. The \u03b2\u2010proteobacterium <jats:italic>Neisseria meningitidis<\/jats:italic> possesses an incomplete PTS unable to transport carbon sources because it lacks a membrane component. Nevertheless, the residual phosphorylation cascade is functional and the meningococcal PTS was therefore expected to carry out regulatory roles. Interestingly, a \u0394<jats:italic>ptsH<\/jats:italic> mutant (lacks the PTS protein HPr) exhibited reduced virulence in mice and after intraperitoneal challenge it was rapidly cleared from the bloodstream of BALB\/c mice. The rapid clearance correlates with lower capsular polysaccharide production by the \u0394<jats:italic>ptsH<\/jats:italic> mutant, which is probably also responsible for its increased adhesion to Hec\u20101\u2010B epithelial cells. In addition, compared to the wild\u2010type strain more apoptotic cells were detected when Hec\u20101\u2010B cells were infected with the \u0394<jats:italic>ptsH<\/jats:italic> strain. Coimmunoprecipitation revealed an interaction of HPr and P\u2010Ser\u2010HPr with the LysR type transcription regulator CrgA, which among others controls its own expression. Moreover, <jats:italic>ptsH<\/jats:italic> deletion caused increased expression of a \u03a6<jats:italic>crgA\u2010lacZ<\/jats:italic> fusion. Finally, the presence of HPr or phospho\u2010HPr's during electrophoretic mobility shift assays enhanced the affinity of CrgA for its target sites preceding <jats:italic>crgA<\/jats:italic> and <jats:italic>pilE<\/jats:italic>, but HPr did not promote CrgA binding to the <jats:italic>sia<\/jats:italic> and <jats:italic>pilC1<\/jats:italic> promoter regions.<\/jats:p>","DOI":"10.1111\/mmi.13349","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T09:05:54Z","timestamp":1455008754000},"page":"788-807","source":"Crossref","is-referenced-by-count":11,"title":["The phosphocarrier protein HPr of <scp><i>N<\/i><\/scp><i>eisseria meningitidis<\/i> interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion, and virulence"],"prefix":"10.1111","volume":"100","author":[{"given":"Meriem","family":"Derkaoui","sequence":"first","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"},{"name":"Institut Pasteur, Unit\u00e9 des Infections Bact\u00e9riennes Invasives 75000 Paris Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Antunes","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 des Infections Bact\u00e9riennes Invasives 75000 Paris Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Poncet","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamila","family":"Nait Abdallah","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"},{"name":"Institut Pasteur, Unit\u00e9 des Infections Bact\u00e9riennes Invasives 75000 Paris Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Joyet","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Maz\u00e9","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9line","family":"Henry","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhamed\u2010Kheir","family":"Taha","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 des Infections Bact\u00e9riennes Invasives 75000 Paris Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Deutscher","sequence":"additional","affiliation":[{"name":"Micalis Institute, INRA, AgroParisTech, Universit\u00e9 Paris\u2010Saclay 78350 Jouy\u2010en\u2010Josas France"},{"name":"Centre National de la Recherche Scientifique, UMR8261, Expression G\u00e9n\u00e9tique Microbienne, Institut de Biologie Physico\u2010Chimique 75005 Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ala\u2010Eddine","family":"Deghmane","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 des Infections Bact\u00e9riennes Invasives 75000 Paris Cedex France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07692.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913017320"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00252-0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00325a023"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90440-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07495.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4166"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000071072"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.04.061"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1000759107"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00763.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03259.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.5.1068"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02838.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.5.2897-2901.2003"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.006221"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000405"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.02.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1984.tb01053.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00263a006"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00369a031"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02280.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00024-06"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00001-14"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb08438.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012679"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.15.3917"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192368699"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.16.8439"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.5.1646"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605010200"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00347.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01923.x"},{"key":"e_1_2_6_35_1","first-page":"121","article-title":"How seryl\u2010phosphorylated HPr inhibits PrfA, a transcription activator of Listeria monocytogenes virulence genes","volume":"9","author":"Herro R.","year":"2005","journal-title":"J Mol Microbiol Biotechnol"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(02)01339-6"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.10.3421-3430.2005"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01687-07"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.42.26530"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05888.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.85.6.1274-1279.1963"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01026-10"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1159\/000233505"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2008\/022772-0"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12522"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.04.022"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00972-06"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03935.x"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212410399"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.2711634.x"},{"key":"e_1_2_6_51_1","volume-title":"Experiments in Molecular Genetics","author":"Miller J.H.","year":"1972"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr500929u"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.11.3391-3398.2001"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.15.3928"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2007.10.002"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.8.2618-2627.2003"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.7.2147-2154.1991"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01615.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02002-07"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1159\/000219374"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/1439-7633(20020603)3:6<490::AID-CBIC490>3.0.CO;2-N"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.11.4836-4842.1997"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(83)90141-1"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp445"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.06.186"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00114"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01071-08"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3744-3751.2002"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01945-06"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.2.475"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/81380"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03335.x"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00876.x"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07098.x"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00033-07"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb00922.x"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00411-15"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl066"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00781-07"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.01955.x"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13349","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13349","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T01:48:21Z","timestamp":1696211301000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13349"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,10]]},"references-count":79,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/mmi.13349"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13349","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,10]]}}}