{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:16:24Z","timestamp":1790676984881,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T00:00:00Z","timestamp":1460592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM059323"],"award-info":[{"award-number":["GM059323"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic> FrvA (Lmo0641) is critical for virulence in the mouse model and is an ortholog of the <jats:italic>Bacillus subtilis<\/jats:italic> Fur\u2010 and PerR\u2010regulated Fe(II) efflux P<jats:sub>1B4<\/jats:sub>\u2010type ATPase PfeT. Previously, FrvA was suggested to protect against heme toxicity. Here, we demonstrate that an <jats:italic>frvA<\/jats:italic> mutant is sensitive to iron intoxication, but not to other metals. Expression of <jats:italic>frvA<\/jats:italic> is induced by high iron and this induction requires Fur. FrvA functions <jats:italic>in vitro<\/jats:italic> as a divalent cation specific ATPase most strongly activated by ferrous iron. When expressed in <jats:italic>B. subtilis<\/jats:italic>, FrvA increases resistance to iron both in wild\u2010type and in a <jats:italic>pfeT<\/jats:italic> null strain. FrvA is a high affinity Fe(II) exporter and its induction imposes severe iron limitation in <jats:italic>B. subtilis<\/jats:italic> resulting in derepression of both Fur\u2010 and PerR\u2010regulated genes. FrvA also recognizes Co(II) and Zn(II) as substrates and can complement <jats:italic>B. subtilis<\/jats:italic> strains defective in the endogenous export systems for these cations. Building on these results, we conclude that FrvA functions in the efflux of Fe(II), and not heme during listerial infection.<\/jats:p>","DOI":"10.1111\/mmi.13368","type":"journal-article","created":{"date-parts":[[2016,3,6]],"date-time":"2016-03-06T08:45:48Z","timestamp":1457253948000},"page":"1066-1079","source":"Crossref","is-referenced-by-count":57,"title":["The <i><scp>L<\/scp>isteria monocytogenes<\/i> Fur\u2010regulated virulence protein FrvA is an Fe(II) efflux P<sub>1B4<\/sub>\u2010type ATPase"],"prefix":"10.1111","volume":"100","author":[{"given":"Hualiang","family":"Pi","sequence":"first","affiliation":[{"name":"Department of Microbiology Cornell University Ithaca NY 14853 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarju J.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Worcester Polytechnic Institute Worcester MA 01609 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