{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T11:51:12Z","timestamp":1770465072945,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,4,20]],"date-time":"2016-04-20T00:00:00Z","timestamp":1461110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Coronafacoyl phytotoxins are secondary metabolites that are produced by various phytopathogenic bacteria, including several pathovars of the Gram\u2010negative bacterium <jats:italic>Pseudomonas syringae<\/jats:italic> as well as the Gram\u2010positive potato scab pathogen <jats:italic>Streptomyces scabies<\/jats:italic>. The phytotoxins are composed of the polyketide coronafacic acid (CFA) linked via an amide bond to amino acids or amino acid derivatives, and their biosynthesis involves the <jats:italic>cfa<\/jats:italic> and <jats:italic>cfa\u2010<\/jats:italic>like gene clusters that are found in <jats:italic>P. syringae<\/jats:italic> and <jats:italic>S. scabies<\/jats:italic>, respectively. The <jats:italic>S. scabies cfa\u2010<\/jats:italic>like gene cluster was previously reported to contain several genes that are absent from the <jats:italic>P. syringae cfa<\/jats:italic> gene cluster, including one (<jats:italic>oxr<\/jats:italic>) encoding a putative F<jats:sub>420<\/jats:sub>\u2014dependent oxidoreductase, and another (<jats:italic>sdr<\/jats:italic>) encoding a predicted short\u2010chain dehydrogenase\/reductase. Using gene deletion analysis, we demonstrated that both <jats:italic>oxr<\/jats:italic> and <jats:italic>sdr<\/jats:italic> are required for normal production of the <jats:italic>S. scabies<\/jats:italic> coronafacoyl phytotoxins, and structural analysis of metabolites that accumulated in the \u0394<jats:italic>sdr<\/jats:italic> mutant cultures revealed that Sdr is directly involved in the biosynthesis of the CFA moiety. Our results suggest that <jats:italic>S. scabies<\/jats:italic> and <jats:italic>P. syringae<\/jats:italic> use distinct biosynthetic pathways for producing coronafacoyl phytotoxins, which are important mediators of host\u2010pathogen interactions in various plant pathosystems.<\/jats:p>","DOI":"10.1111\/mmi.13378","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T07:06:41Z","timestamp":1458371201000},"page":"122-135","source":"Crossref","is-referenced-by-count":15,"title":["Production of the <i>Streptomyces scabies<\/i> coronafacoyl phytotoxins involves a novel biosynthetic pathway with an F<sub>420<\/sub>\u2010dependent oxidoreductase and a short\u2010chain dehydrogenase\/reductase"],"prefix":"10.1111","volume":"101","author":[{"given":"Luke","family":"Bown","sequence":"first","affiliation":[{"name":"Department of Biology Memorial University of Newfoundland St. John's NL A1B 3X9 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mead S.","family":"Altowairish","sequence":"additional","affiliation":[{"name":"Department of Biology Memorial University of Newfoundland St. John's NL A1B 3X9 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna K.","family":"Fyans","sequence":"additional","affiliation":[{"name":"Department of Biology Memorial University of Newfoundland St. John's NL A1B 3X9 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn R. 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