{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:02:13Z","timestamp":1791338533522,"version":"4.3.1"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,6,21]],"date-time":"2016-06-21T00:00:00Z","timestamp":1466467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Mycobacterium tuberculosis<\/jats:italic> (<jats:italic>Mtb<\/jats:italic>) causes latent tuberculosis infection in one\u2010third of the world population and remains quiescent in the human body for decades. The dormant pathogen accumulates lipid droplets containing triacylglycerol (TAG). In mammals, perilipin regulates lipid droplet homeostasis but no such protein has been identified in <jats:italic>Mtb<\/jats:italic>. We identified an <jats:italic>Mtb<\/jats:italic> protein (PPE15) that showed weak amino acid sequence identities with mammalian perilipin\u20101 and was upregulated in <jats:italic>Mtb<\/jats:italic> dormancy. We generated a <jats:italic>ppe15<\/jats:italic> gene\u2010disrupted mutant of <jats:italic>Mtb<\/jats:italic> and examined its ability to metabolically incorporate radiolabeled oleic acid into TAG, accumulate lipid droplets containing TAG and develop phenotypic tolerance to rifampicin in two <jats:italic>in vitro<\/jats:italic> models of dormancy including a three\u2010dimensional human granuloma model. The mutant showed a significant decrease in the biosynthesis and accumulation of lipid droplets containing TAG and in its tolerance of rifampicin. Complementation of the mutant with a wild\u2010type copy of the <jats:italic>ppe15<\/jats:italic> gene restored the lost phenotypes. We designate PPE15 as mycobacterial perilipin\u20101 (MPER1). Our findings suggest that the MPER1 protein plays a critical role in the homeostasis of TAG \u2010containing lipid droplets in <jats:italic>Mtb<\/jats:italic> and influences the entry of the pathogen into a dormant state.<\/jats:p>","DOI":"10.1111\/mmi.13422","type":"journal-article","created":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T11:20:22Z","timestamp":1466594422000},"page":"784-794","source":"Crossref","is-referenced-by-count":49,"title":["The perilipin\u2010like PPE15 protein in <i>Mycobacterium tuberculosis<\/i> is required for triacylglycerol accumulation under dormancy\u2010inducing conditions"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3605-687X","authenticated-orcid":false,"given":"Jaiyanth","family":"Daniel","sequence":"first","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine University of Central Florida Orlando FL 32816 USA"},{"name":"Department of Biology Indiana University\u2010Purdue University Fort Wayne Fort Wayne IN 46805 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Kapoor","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine University of Central Florida Orlando FL 32816 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Sirakova","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine University of Central Florida Orlando FL 32816 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Sinha","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine University of Central Florida Orlando FL 32816 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pappachan","family":"Kolattukudy","sequence":"additional","affiliation":[{"name":"Burnett School of Biomedical Sciences, College of Medicine 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.023028-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01001-15"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-10-3007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.04.002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-008-9998-8"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.05004-11"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.204966"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.5017-5030.2004"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002093"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114877"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M505556200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0006077"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2011.01.001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063814"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12981"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050075"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.003"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053657"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013938"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.445056"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00530-09"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.135731"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005351"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00410-07"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-7-84"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2005.08.016"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.MGM2-0010-2013"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000204"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12092"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01670-06"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-249"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M900176-JLR200"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28993-0"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051641"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004183"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M400291-JLR200"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2004.02.003"},{"key":"e_1_2_6_39_1","unstructured":"World 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