{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:29:56Z","timestamp":1792798196341,"version":"4.5.0"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"U.S. National Institutes of Allergy and Infectious Diseases","award":["R21AI111317 (L.T.H and T.L), R21AI103905 (L.T.H. and T.L) and F32AI098388 (E.B.T.)"],"award-info":[{"award-number":["R21AI111317 (L.T.H and T.L), R21AI103905 (L.T.H. and T.L) and F32AI098388 (E.B.T.)"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    maintains a complex life cycle between tick and vertebrate hosts. Although some genes have been identified as contributing to bacterial adaptation in the different hosts, the list is incomplete. In this manuscript, we report the first use of transposon mutagenesis combined with high\u2010throughput sequencing (Tn\u2010seq) in\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    . We utilize the technique to investigate mechanisms of carbohydrate utilization in\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    and the role of carbohydrate metabolism during mouse infection. We performed genetic fitness analyses to identify genes encoding factors contributing to growth on glucose, maltose, mannose, trehalose and\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acetyl\u2010glucosamine. We obtained insight into the potential functions of proteins predicted to be involved in carbohydrate utilization and identified additional factors previously unrecognized as contributing to the metabolism of the tested carbohydrates. Strong phenotypes were observed for the putative carbohydrate phosphotransferase transporters BB0408 and BBB29 as well as the response regulator Rrp1. We further validated Tn\u2010seq for use in mouse studies and were able to correctly identify known infectivity factors as well as additional transporters and genes on lp54 that may contribute to optimal mouse infection. As such, this study establishes Tn\u2010seq as a powerful method for both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/mmi.13437","type":"journal-article","created":{"date-parts":[[2016,6,9]],"date-time":"2016-06-09T06:48:54Z","timestamp":1465454934000},"page":"1003-1023","source":"Crossref","is-referenced-by-count":40,"title":["Global Tn\u2010seq analysis of carbohydrate utilization and vertebrate infectivity of\n                    <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1111","volume":"101","author":[{"given":"Erin B.","family":"Troy","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Texas Medical Center at Houston Houston TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihui","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Texas Medical Center at Houston Houston TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Lazinski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maureen","family":"Lundt","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Camilli","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine Boston MA"},{"name":"Howard Hughes Medical Institute Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Norris","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Texas Medical Center at Houston Houston TX"},{"name":"Department of Microbiology and Molecular Genetics University of Texas Medical Center at Houston Houston TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linden T.","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Microbiology Tufts University School of Medicine Boston 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