{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T17:06:36Z","timestamp":1769015196424,"version":"3.49.0"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,7,18]],"date-time":"2016-07-18T00:00:00Z","timestamp":1468800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01 AI047141"],"award-info":[{"award-number":["R01 AI047141"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Neisseria gonorrhoeae<\/jats:italic> produces two transferrin binding proteins, TbpA and TbpB, which together enable efficient iron transport from human transferrin. We demonstrate that expression of the <jats:italic>tbp<\/jats:italic> genes is controlled by MisR, a response regulator in the two\u2010component regulatory system that also includes the sensor kinase MisS. The <jats:italic>tbp<\/jats:italic> genes were up\u2010regulated in the <jats:italic>misR<\/jats:italic> mutant under iron\u2010replete conditions but were conversely down\u2010regulated in the <jats:italic>misR<\/jats:italic> mutant under iron\u2010depleted conditions. The <jats:italic>misR<\/jats:italic> mutant was capable of transferrin\u2010iron uptake at only 50% of wild\u2010type levels, consistent with decreased <jats:italic>tbp<\/jats:italic> expression. We demonstrate that phosphorylated MisR specifically binds to the <jats:italic>tbpBA<\/jats:italic> promoter and that MisR interacts with five regions upstream of the <jats:italic>tbpB<\/jats:italic> start codon. These analyses confirm that MisR directly regulates <jats:italic>tbpBA<\/jats:italic> expression. The MisR binding sites in the gonococcus are only partially conserved in <jats:italic>Neisseria meningitidis<\/jats:italic>, which may explain why <jats:italic>tbpBA<\/jats:italic> was not MisR\u2010regulated in previous studies using this related pathogen. This is the first report of a <jats:italic>trans<\/jats:italic>\u2010acting protein factor other than Fur that can directly contribute to gonococcal <jats:italic>tbpBA<\/jats:italic> regulation.<\/jats:p>","DOI":"10.1111\/mmi.13450","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T09:09:28Z","timestamp":1467191368000},"page":"137-151","source":"Crossref","is-referenced-by-count":7,"title":["The genes that encode the gonococcal transferrin binding proteins, TbpB and TbpA, are differentially regulated by MisR under iron\u2010replete and iron\u2010depleted conditions"],"prefix":"10.1111","volume":"102","author":[{"given":"Justin L.","family":"Kandler","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Emory University School of Medicine Atlanta Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosuany V\u00e9lez","family":"Acevedo","sequence":"additional","affiliation":[{"name":"Department of 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