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With the exception of UAP56, a component of TREX (<jats:styled-content>Tr<\/jats:styled-content>anscription <jats:styled-content>Ex<\/jats:styled-content>port) complex, other components of mRNA export machinery are not well conserved in divergent supergroups. Here, we use <jats:italic>Toxoplasma gondii<\/jats:italic> as a model system to functionally characterize TgUAP56 and its potential interaction factors. We demonstrate that TgUAP56 is crucial for mRNA export and that functional interference leads to significant accumulation of mRNA in the nucleus. It was necessary to employ bioinformatics and phylogenetic analysis to identify orthologs related to mRNA export, which show a remarkable low level of conservation in <jats:italic>T. gondii<\/jats:italic>. We adapted a conditional Cas9\/CRISPR system to carry out a genetic screen to verify if these factors were involved in mRNA export in <jats:italic>T. gondii<\/jats:italic>. Only the disruption of TgRRM_1330 caused accumulation of mRNA in the nucleus as found with TgUAP56. This protein is potentially a divergent partner of TgUAP56, and provides insight into a divergent mRNA export pathway in apicomplexans.<\/jats:p>","DOI":"10.1111\/mmi.13485","type":"journal-article","created":{"date-parts":[[2016,8,20]],"date-time":"2016-08-20T09:02:54Z","timestamp":1471683774000},"page":"672-689","source":"Crossref","is-referenced-by-count":24,"title":["UAP56 is a conserved crucial component of a divergent mRNA export pathway in <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"102","author":[{"given":"Mariana","family":"Serpeloni","sequence":"first","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ Curitiba Brazil"},{"name":"Departamento de Biologia Celular e Molecular Universidade Federal do Paran\u00e1 Curitiba Brazil"},{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Jim\u00e9nez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Newton Medeiros","family":"Vidal","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constanze","family":"Kroeber","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Andenmatten","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro","family":"Lemgruber","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"M\u00f6rking","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gurman S.","family":"Pall","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Meissner","sequence":"additional","affiliation":[{"name":"College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity &amp; Inflammation, Wellcome Trust Centre for Molecular Parasitology, University of Glasgow  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9a R.","family":"\u00c1vila","sequence":"additional","affiliation":[{"name":"Instituto Carlos Chagas, FIOCRUZ Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2301"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.025"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3552"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.01.039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv330"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00268"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.5258504"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2009.07.017"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells4030452"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1103"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","DOI":"10.1074\/jbc.271.24.14010","article-title":"Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine\u2010xanthine\u2010guanine phosphoribosyltransferase gene. 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