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Phagocytosis of bacteria by the amebic trophozoite has been reported to be important for the virulence of the parasite. Here, we set out to characterize different stages of phagocytosis of type 1 <jats:italic>E. coli<\/jats:italic> and investigated the role of a set of amoebic Rab GTPases in the process. The localizations of the Rab GTPases during different stages of the phagocytosis were investigated using laser scanning confocal microscopy and their functional relevance were determined using fluorescence activated cell sorter based assay as well as colony forming unit assay. Our results demonstrate that EhRab7A is localized on the phagosomes and involved in both early and late stages of type 1 <jats:italic>E. coli<\/jats:italic> phagocytosis. We further showed that the <jats:italic>E. coli<\/jats:italic> or RBC containing phagosomes are distinct from the large endocytic vacuoles in the parasite which are exclusively used to transport human holotransferrin and low density lipoprotein. Remarkably, type 1 <jats:italic>E. coli<\/jats:italic> uptake was found to be insensitive to cytochalasin D treatment, suggesting that the initial stage of <jats:italic>E. coli<\/jats:italic> phagocytosis is independent of the formation of actin filaments.<\/jats:p>","DOI":"10.1111\/mmi.13533","type":"journal-article","created":{"date-parts":[[2016,9,24]],"date-time":"2016-09-24T10:16:07Z","timestamp":1474712167000},"page":"1043-1061","source":"Crossref","is-referenced-by-count":11,"title":["Role of EhRab7A in phagocytosis of type 1 fimbriated <i>E. coli<\/i> by <i>Entamoeba histolytica<\/i>"],"prefix":"10.1111","volume":"102","author":[{"given":"Kuldeep","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Biological Science Indian Institute of Science Education and Research Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyoshi","family":"Nozaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology National Institute of Infectious Diseases  Tokyo 162\u20108640 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunando","family":"Datta","sequence":"additional","affiliation":[{"name":"Department of Biological Science Indian Institute of Science Education and Research Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,20]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"WHO(1997) WHO\/PAHO\/UNESCO report. 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