{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:18:56Z","timestamp":1789607936315,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,16]],"date-time":"2016-12-16T00:00:00Z","timestamp":1481846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Upon sensing of the peptide pheromone cCF10,\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    cells carrying pCF10 produce three surface adhesins (PrgA, PrgB or Aggregation Substance, PrgC) and the Prg\/Pcf type IV secretion system and, in turn, conjugatively transfer the plasmid at high frequencies to recipient cells. Here, we report that cCF10 induction is highly toxic to cells sustaining a deletion of\n                    <jats:italic>prgU<\/jats:italic>\n                    , a small\n                    <jats:italic>orf<\/jats:italic>\n                    located immediately downstream of\n                    <jats:italic>prgB<\/jats:italic>\n                    on pCF10. Upon pheromone exposure, these cells overproduce the Prg adhesins and display impaired envelope integrity, as evidenced by antibiotic susceptibility, misplaced division septa and cell lysis. Compensatory mutations in regulatory loci controlling expression of pCF10\u2010encoded\n                    <jats:italic>prg\/pcf<\/jats:italic>\n                    genes, or constitutive PrgU overproduction, block production of the Prg adhesins and render cells insensitive to pheromone. Cells engineered to overproduce PrgB, even independently of other pCF10\u2010encoded proteins, have severely compromised cell envelopes and strong growth defects. PrgU has an RNA\u2010binding fold, and\n                    <jats:italic>prgB<\/jats:italic>\n                    \u2010\n                    <jats:italic>prgU<\/jats:italic>\n                    gene pairs are widely distributed among\n                    <jats:italic>E. faecalis<\/jats:italic>\n                    isolates and other enterococcal and staphylococcal species. Together, our findings support a model in which PrgU proteins represent a novel class of RNA\u2010binding regulators that act to mitigate toxicity accompanying overproduction of PrgB\u2010like adhesins in\n                    <jats:italic>E.\u00a0faecalis<\/jats:italic>\n                    and other clinically\u2010important Gram\u2010positive species.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13563","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T14:48:51Z","timestamp":1479307731000},"page":"398-412","source":"Crossref","is-referenced-by-count":31,"title":["<scp>P<\/scp>\n                    rg\n                    <scp>U<\/scp>\n                    : a suppressor of sex pheromone toxicity in\n                    <scp>\n                      <i>E<\/i>\n                    <\/scp>\n                    <i>nterococcus faecalis<\/i>"],"prefix":"10.1111","volume":"103","author":[{"given":"Minny","family":"Bhatty","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics McGovern Medical School 6431 Fannin St Houston Texas 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha I.","family":"Camacho","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics McGovern Medical School 6431 Fannin St Houston Texas 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Gonzalez\u2010Rivera","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics McGovern Medical School 6431 Fannin St Houston Texas 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristi L.","family":"Frank","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Minnesota Medical School, Microbiology Research Facility 689 23rd Ave, S.E Minneapolis Minnesota 55455 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Dale","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Minnesota Medical School, Microbiology Research Facility 689 23rd Ave, S.E Minneapolis Minnesota 55455 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn A.","family":"Manias","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Minnesota Medical School, Microbiology Research Facility 689 23rd Ave, S.E Minneapolis Minnesota 55455 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary M.","family":"Dunny","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Minnesota Medical School, Microbiology Research Facility 689 23rd Ave, S.E Minneapolis Minnesota 55455 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Christie","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics McGovern Medical School 6431 Fannin St Houston Texas 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03832.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00193-12"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3311709.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3301710.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12893"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","unstructured":"Chang C. Volkart L. Moy S. andJoachimiak A.(2006) Crystal structure of protein EF0006 fromEnterococcus faecalis. In RCSV Protein Data Bank: Midwest Center for Structural Genomics.","DOI":"10.2210\/pdb2gmq\/pdb"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1212256110"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101569108"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05610.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01999-07"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.11.5161-5168.1988"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01034-08"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9020"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05757.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2007.05.001"},{"key":"e_1_2_6_17_1","volume-title":"Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet]","author":"Clewell D.B.","year":"2014"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12046"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07786.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00344-15"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0554-6_12"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03984-14"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00926-12"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-111212-133449"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00128-16"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.124.2.784-790.1975"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.01.008"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.24.8582"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.4.1194-1201.1991"},{"key":"e_1_2_6_30_1","volume-title":"Enterococci: From Commensals to Leading Causes of Drug Resistant Infection","author":"Dunny G.M.","year":"2014"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05964-11"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1500553112"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.3.1044-1054.2005"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01525-09"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1319066110"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.2"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01837-08"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.01.040"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05434.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2006.08.003"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00950-13"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00534-13"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004380050315"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01176"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00648-12"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13081"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000582"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.23.7405-7408.1994"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/007252-0"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.216-222.1996"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.23.7665-7672.1991"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.19.6229-6237.1993"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2010.08.004"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004056"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00513-06"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.06031.x"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.6067-6075.1999"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140784"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512841200"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.218-223.1998"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2010.03.002"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.24198"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880903200902"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06703.x"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091213-113003"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.7.2169-2177.2003"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12780"},{"key":"e_1_2_6_68_1","first-page":"576","article-title":"Insights into the catalytic mechanism of 16S rRNA methyltransferase RsmE (m3U1498) from crystal and solution structures","volume":"423","author":"Zhang H.","year":"2012","journal-title":"J Mol Microbiol"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.13563","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13563","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T18:51:24Z","timestamp":1692557484000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13563"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,16]]},"references-count":67,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/mmi.13563"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13563","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,16]]}}}