{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:36:21Z","timestamp":1790476581606,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T00:00:00Z","timestamp":1483920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LZ15C010001"],"award-info":[{"award-number":["LZ15C010001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Antibiotic\u2010producing microorganisms have evolved several self\u2010resistance mechanisms to prevent auto\u2010toxicity. Overexpression of specific transporters to improve the efflux of toxic antibiotics has been found one of the most important and intrinsic resistance strategies used by many <jats:italic>Streptomyces<\/jats:italic> strains. In\u00a0this work, two ATP\u2010binding cassette (ABC) transporter\u2010encoding genes located in the natamycin biosynthetic gene cluster, <jats:italic>scnA<\/jats:italic> and <jats:italic>scnB<\/jats:italic>, were identified as the primary exporter genes for natamycin efflux in <jats:italic>Streptomyces chattanoogensis<\/jats:italic> L10. Two other transporters located outside the cluster, a major facilitator superfamily transporter Mfs1 and an ABC transporter NepI\/II were found to play a complementary role in natamycin efflux. ScnA\/ScnB and Mfs1 also participate in exporting the immediate precursor of natamycin, 4,5\u2010de\u2010epoxynatamycin, which is more toxic to <jats:italic>S. chattanoogensis<\/jats:italic> L10 than natamycin. As the major complementary exporter for natamycin efflux, Mfs1 is up\u2010regulated in response to intracellular accumulation of natamycin and 4,5\u2010de\u2010epoxynatamycin, suggesting a key role in the stress response for self\u2010resistance. This article discusses a novel antibiotic\u2010related efflux and response system in <jats:italic>Streptomyces<\/jats:italic>, as well as a self\u2010resistance mechanism in antibiotic\u2010producing strains.<\/jats:p>","DOI":"10.1111\/mmi.13583","type":"journal-article","created":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T12:35:49Z","timestamp":1479818149000},"page":"713-728","source":"Crossref","is-referenced-by-count":26,"title":["Multiple transporters are involved in natamycin efflux in <scp><i>S<\/i><\/scp><i>treptomyces chattanoogensis<\/i> L10"],"prefix":"10.1111","volume":"103","author":[{"given":"Tan\u2010Jun","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ming","family":"Shan","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Wang","family":"Dou","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Hang","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu\u2010Ming","family":"Mao","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"},{"name":"Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolism Engineering 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shui\u2010Ping","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jun","family":"Guan","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"},{"name":"Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolism Engineering 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Quan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, College of Pharmaceutical Sciences, Zhejiang University 866 Yuhangtang Road Hangzhou 310058 China"},{"name":"Zhejiang Provincial Key Laboratory for Microbial Biochemistry and Metabolism Engineering 866 Yuhangtang Road Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/009126-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.15.10133"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(00)00120-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(01)00046-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200402"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2003.10.007"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-010-0721-x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05155"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)31588-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-009-0014-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.046607-0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2006.00060.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1128\/JB.180.16.4017-4023.1998","article-title":"A silent ABC transporter isolated from Streptomyces rochei F20 induces multidrug resistance","volume":"180","author":"Fernandez\u2010Moreno M.A.","year":"1998","journal-title":"J Bacteriol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.061325-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100822"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0337542100"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05584.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.28.17933"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.61.080706.093329"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06735.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-6307-1"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2015.01.013"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12689"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(09)04610-2"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.04.009"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-5521(01)00033-3"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20040490"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2007.01.010"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2003.12.010"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02305.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.01.005"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.69.2.326-356.2005"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2015.06.007"},{"key":"e_1_2_6_37_1","first-page":"406","article-title":"The neighbor\u2010joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou N.","year":"1987","journal-title":"Mol Biol Evol"},{"key":"e_1_2_6_38_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2001"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4576-4583.2005"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05559.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-144-12-3351"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0900592106"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100345"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv437"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03003-13"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12959"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00171-2"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-013-1370-7"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00191-12"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12406"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402577"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13583","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T18:19:55Z","timestamp":1696184395000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,9]]},"references-count":50,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/mmi.13583"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13583","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,9]]}}}