{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:28Z","timestamp":1791942388240,"version":"4.3.4"},"reference-count":64,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T00:00:00Z","timestamp":1484006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    is a pathogen infecting cattle, able to survive, traffic, and proliferate inside host cells. It belongs to the\n                    <jats:italic>Alphaproteobacteria<\/jats:italic>\n                    , a phylogenetic group comprising bacteria with free living, symbiotic, and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium\n                    <jats:italic>Caulobacter crescentus<\/jats:italic>\n                    . This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP\u2010seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional\n                    <jats:italic>B. abortus ctrA<\/jats:italic>\n                    loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of\n                    <jats:italic>B. abortus<\/jats:italic>\n                    in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the\n                    <jats:italic>B. abortus<\/jats:italic>\n                    envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13589","type":"journal-article","created":{"date-parts":[[2016,11,28]],"date-time":"2016-11-28T10:27:12Z","timestamp":1480328832000},"page":"780-797","source":"Crossref","is-referenced-by-count":45,"title":["CtrA controls cell division and outer membrane composition of the pathogen\n                    <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"103","author":[{"given":"Nayla","family":"Francis","sequence":"first","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katy","family":"Poncin","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Fioravanti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle UMR 8576 CNRS \u2013 Universit\u00e9 de Lille 50 Avenue Halley Villeneuve d'Ascq France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Vassen","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Willemart","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thi Anh Phuong","family":"Ong","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Rappez","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Jacques","family":"Letesson","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele G.","family":"Biondi","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Glycobiologie Structurale et Fonctionnelle UMR 8576 CNRS \u2013 Universit\u00e9 de Lille 50 Avenue Halley Villeneuve d'Ascq France"},{"name":"Laboratoire de Chimie Bact\u00e9rienne Institut de Microbiologie de la M\u00e9diterran\u00e9e, Aix\u2010Marseille Universit\u00e9, CNRS UMR 7283 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"De Bolle","sequence":"additional","affiliation":[{"name":"Microorganisms Biology Research Unit (URBM) Narilis, University of Namur Namur Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.6.2240-2246.1998"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00444-09"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040497"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02777.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1752-0509-4-52"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1114476109"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406873102"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00221.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07885.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-138-7-1543"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00102.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00040-09"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.09.007"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5366"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2001.00131.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.58.7.2320-2328.1990"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80502-4"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00749.x"},{"key":"e_1_2_7_21_1","first-page":"311","article-title":"Evidence of three major polypeptide species and two major polysaccharide species in the Brucella outer membrane","volume":"14","author":"Dubray G.","year":"1983","journal-title":"Ann Vet Res"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1461"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13011"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5081"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.2873-2880.2005"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05571.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1515544112"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00170-12"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.06.002"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01873-06"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601577"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/367654a0"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095191"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4823-4830.2001"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01098-08"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.2.859"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.062065699"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00712-06"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00025-08"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00439-07"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.16.5631-5639.2005"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251216598"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2012.02553.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00066-10"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-30745-1_17"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005232"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12347"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5711-5724.1998"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2387-2392.1998"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80995-2"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.1.120"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.8.2430-2439.1999"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.12.3482-3489.2000"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit007"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604087200"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07579.x"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.12.002"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00718.x"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1304080110"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082514"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.10.008"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06357-11"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80379-2"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1503118112"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1007"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13589","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13589","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T22:38:46Z","timestamp":1696199926000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13589"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,10]]},"references-count":64,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/mmi.13589"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13589","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,10]]}}}