{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T20:12:42Z","timestamp":1774901562978,"version":"3.50.1"},"reference-count":66,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Campylobacter jejuni<\/jats:italic> helical shape is important for colonization and host interactions with straight mutants having altered biological properties. Passage on calcofluor white (CFW) resulted in <jats:italic>C. jejuni<\/jats:italic> 81\u2010176 isolates with morphology changes: either a straight morphology from frameshift mutations and single nucleotide polymorphisms in peptidoglycan hydrolase genes <jats:italic>pgp1<\/jats:italic> or <jats:italic>pgp2<\/jats:italic> or a reduction in curvature due a frameshift mutation in <jats:italic>cjj81176_1105<\/jats:italic>, a putative peptidoglycan endopeptidase. Shape defects were restored by complementation. Whole genome sequencing of CFW\u2010passaged strains showed no specific changes correlating to CFW exposure. The <jats:italic>cjj81176_1279<\/jats:italic> (<jats:italic>recR<\/jats:italic>; recombinational DNA repair) and <jats:italic>cjj81176_1449<\/jats:italic> (unknown function) genes were highly variable in all 81\u2010176 strains sequenced. A frameshift mutation in <jats:italic>pgp1<\/jats:italic> of our laboratory isolate of the straight genome sequenced variant of 11168 (11168\u2010GS) was also identified. The PG muropeptide profile of 11168\u2010GS was identical to that of \u0394<jats:italic>pgp1<\/jats:italic> in the original minimally passaged 11168 strain (11168\u2010O). Introduction of wild type <jats:italic>pgp1<\/jats:italic> into 11168\u2010GS did not restore helical morphology. The <jats:italic>recR<\/jats:italic> gene was also highly variable in 11168 strains. Microbial cell\u2010to\u2010cell heterogeneity is proposed as a mechanism of ensuring bacterial survival in sub\u2010optimal conditions. In certain environments, changes in <jats:italic>C. jejuni<\/jats:italic> morphology due to genetic heterogeneity may promote <jats:italic>C. jejuni<\/jats:italic> survival.<\/jats:p>","DOI":"10.1111\/mmi.13672","type":"journal-article","created":{"date-parts":[[2017,3,19]],"date-time":"2017-03-19T05:58:58Z","timestamp":1489903138000},"page":"948-971","source":"Crossref","is-referenced-by-count":21,"title":["Morphology heterogeneity within a <i>Campylobacter jejuni<\/i> helical population: the use of calcofluor white to generate rod\u2010shaped <i>C. jejuni<\/i> 81\u2010176 clones and the genetic determinants responsible for differences in morphology within 11168 strains"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9710-9410","authenticated-orcid":false,"given":"Emilisa","family":"Frirdich","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology University of British Columbia Vancouver BC Canada V6T 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