{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:24:48Z","timestamp":1790529888435,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T00:00:00Z","timestamp":1492992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100013281","name":"JPIAMR","doi-asserted-by":"crossref","award":["ANR-15- JAMR-0003-02"],"award-info":[{"award-number":["ANR-15- JAMR-0003-02"]}],"id":[{"id":"10.13039\/100013281","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The predicted shortage in new antibiotics has prompted research for chemicals that could act as adjuvant and enhance efficacy of available antibiotics. In this study, we tested the effects of combining metals with aminoglycosides on <jats:italic>Escherichia coli<\/jats:italic> survival. The best synergizing combination resulted from mixing aminoglycosides with silver. Using genetic and aminoglycoside uptake assays, we showed that silver potentiates aminoglycoside action in by\u2010passing the PMF\u2010dependent step, but depended upon protein translation. We showed that oxidative stress or Fe\u2013S cluster destabilization were not mandatory factors for silver potentiating action. Last, we showed that silver allows aminoglycosides to kill an <jats:italic>E. coli<\/jats:italic> gentamicin resistant mutant as well as the highly recalcitrant anaerobic pathogen <jats:italic>Clostridium difficile<\/jats:italic>. Overall this study delineates the molecular basis of silver's potentiating action on aminoglycoside toxicity and shows that use of metals might offer solutions for battling against increased bacterial resistance to antibiotics.<\/jats:p>","DOI":"10.1111\/mmi.13687","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T10:37:32Z","timestamp":1491475052000},"page":"115-126","source":"Crossref","is-referenced-by-count":42,"title":["Silver potentiates aminoglycoside toxicity by enhancing their uptake"],"prefix":"10.1111","volume":"105","author":[{"given":"Marion","family":"Herisse","sequence":"first","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, Institut de Microbiologie de la M\u00e9diterran\u00e9e Aix\u2010Marseille Universit\u00e9, CNRS, UMR 7283 31 Chemin Joseph Aiguier 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohann","family":"Duverger","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, Institut de Microbiologie de la M\u00e9diterran\u00e9e Aix\u2010Marseille Universit\u00e9, CNRS, UMR 7283 31 Chemin Joseph Aiguier 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Martin\u2010Verstraete","sequence":"additional","affiliation":[{"name":"Institut Pasteur 25\u201028, rue du Docteur Roux Laboratoire Pathogen\u00e8se des Bact\u00e9ries Ana\u00e9robies 75724 Paris Cedex 15 France"},{"name":"Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3458-2574","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Barras","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, Institut de Microbiologie de la M\u00e9diterran\u00e9e Aix\u2010Marseille Universit\u00e9, CNRS, UMR 7283 31 Chemin Joseph Aiguier 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Ezraty","sequence":"additional","affiliation":[{"name":"Laboratoire de Chimie Bact\u00e9rienne, Institut de Microbiologie de la M\u00e9diterran\u00e9e Aix\u2010Marseille Universit\u00e9, CNRS, UMR 7283 31 Chemin Joseph Aiguier 13009 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10069"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/107662904323047817"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.15.1.7"},{"key":"e_1_2_8_5_1","first-page":"1048","article-title":"Effect of streptomycin on Escherichia coli: uptake of streptomycin by Escherichia coli","volume":"187","author":"Davis B.D.","year":"1960","journal-title":"Nature"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.51.3.341-350.1987"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuw004"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1238328"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3"},{"key":"e_1_2_8_10_1","first-page":"168","article-title":"Role of ribosome recycling in uptake of dihydrostreptomycin by sensitive and resistant Escherichia coli","volume":"652","author":"Hurwitz C.","year":"1981","journal-title":"BBA Sect Nucleic Acids Protein Synth"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45790-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu286"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/7.3.229"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1408913"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1232751"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.7.4278-4281.2003"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3MT00359K"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3006276"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.119.3.736-747.1974"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2007.07.004"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2164"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145748"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.1.1.54"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01781-12"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.51.4.439-457.1987"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06954.x"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000497"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.12.006"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07368-11"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13687","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13687","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.13687","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13687","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:50:22Z","timestamp":1693997422000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,24]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1111\/mmi.13687"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13687","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,24]]}}}