{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:16:26Z","timestamp":1740125786068,"version":"3.37.3"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,5,30]],"date-time":"2017-05-30T00:00:00Z","timestamp":1496102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Through <jats:italic>Minos<\/jats:italic> transposon mutagenesis we obtained <jats:italic>A. nidulans<\/jats:italic> mutants resistant to 5\u2010fluorouracil due to insertions into the upstream region of the uncharacterized gene <jats:italic>nmeA<\/jats:italic>, encoding a Major Facilitator Superfamily (MFS) transporter. <jats:italic>Minos<\/jats:italic> transpositions increased <jats:italic>nmeA<\/jats:italic> transcription, which is otherwise extremely low under all conditions tested. To dissect the function of NmeA we used strains overexpressing or genetically lacking the <jats:italic>nmeA<\/jats:italic> gene. Strains overexpressing NmeA are resistant to toxic purine analogues, but also, to cadmium, zinc and borate, whereas an isogenic <jats:italic>nmeA\u0394<\/jats:italic> null mutant exhibits increased sensitivity to these compounds. We provide direct evidence that <jats:italic>nmeA<\/jats:italic> overexpression leads to efflux of adenine, xanthine, uric acid and allantoin, the latter two being intermediate metabolites of purine catabolism that are toxic when accumulated cytoplasmically due to relevant genetic lesions. By using a functional GFP\u2010tagged version we show that NmeA is a plasma membrane transporter. Homology modeling and docking approaches identified a single purine binding site and a tentative substrate translocation trajectory in NmeA. Orthologues of NmeA are present in all Aspergilli and other Eurotiomycetes, but are absent from other fungi or non\u2010fungal organisms. NmeA is thus the founding member of a new class of transporters essential for fungal success under specific toxic conditions.<\/jats:p>","DOI":"10.1111\/mmi.13708","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T10:22:49Z","timestamp":1494930169000},"page":"426-439","source":"Crossref","is-referenced-by-count":6,"title":["NmeA, a novel efflux transporter specific for nucleobases and nucleosides, contributes to metal resistance in <i>Aspergillus nidulans<\/i>"],"prefix":"10.1111","volume":"105","author":[{"given":"Sofia","family":"Balaska","sequence":"first","affiliation":[{"name":"Department of Biology National and Kapodistrian University of Athens Panepistimioupolis Athens 15784 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vassilios","family":"Myrianthopoulos","sequence":"additional","affiliation":[{"name":"Department of Pharmacy National and Kapodistrian University of Athens Panepistimioupolis Athens 15771 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"Tselika","sequence":"additional","affiliation":[{"name":"Department of Biology National and Kapodistrian University of Athens Panepistimioupolis Athens 15784 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitris G.","family":"Hatzinikolaou","sequence":"additional","affiliation":[{"name":"Department of Biology National and Kapodistrian University of Athens Panepistimioupolis Athens 15784 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Mikros","sequence":"additional","affiliation":[{"name":"Department of Pharmacy National and Kapodistrian University of Athens Panepistimioupolis Athens 15771 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3426-726X","authenticated-orcid":false,"given":"George","family":"Diallinas","sequence":"additional","affiliation":[{"name":"Department of Biology National and Kapodistrian University of Athens Panepistimioupolis Athens 15784 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2010.02187.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1205532110"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10635150600755453"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20150047"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ic900176c"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-09-14-0264-R"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/psb.6304"},{"key":"e_1_2_6_9_1","first-page":"107","volume-title":"Aspergillus fumigatus and Aspergillosis","author":"Calera J.A.","year":"2008"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2004.06.007"},{"volume-title":"The Aspergilli: Genomics, Medicine, Biotechnology and Research Methods","year":"2008","author":"Diallinas G.","key":"e_1_2_6_11_1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1001-0742(08)60032-7"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.05.007"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2004.12.006"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2014.06.005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01808-12"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(199701)13:1<43::AID-YEA56>3.0.CO;2-J"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06997.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.2323\/jgam.49.63"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2015.94"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2013.10.009"},{"key":"e_1_2_6_22_1","first-page":"30187","article-title":"Biochemical studies on the structure\u2010 function relationship of major drug transporters in the ATP\u2010 binding cassette family and solute carrier family","volume":"15","author":"Hong M.","year":"2016","journal-title":"Adv Drug Deliv Rev"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1087619"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2016.05.011"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419325112"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394390-3.00008-2"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00261172"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12184"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041939"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(03)00208-2"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2013.12.004"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.01.009"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12982"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509854112"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12284"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.2845"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400336111"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0246"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4232-7"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920210790886871"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004272227886"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.105.052563"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00347.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1004276311956"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcw162"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00083.x"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00143"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-s1-s2"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2014.00202"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2015.25"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar0302843"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1103"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340230306"},{"volume-title":"Molecular Cloning: A Laboratory Manual","year":"1989","author":"Sambrook J.","key":"e_1_2_6_54_1"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-014-0751-0"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13559"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07729.x"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/24.4.649"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.00246"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00556.x"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20150139"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12218"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2013.11.002"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-060414-033901"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02422.x"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2759"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13708","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T06:25:40Z","timestamp":1696227940000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,30]]},"references-count":65,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/mmi.13708"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13708","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"type":"print","value":"0950-382X"},{"type":"electronic","value":"1365-2958"}],"subject":[],"published":{"date-parts":[[2017,5,30]]}}}