{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:16:19Z","timestamp":1787015779557,"version":"build-2736575974"},"reference-count":90,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T00:00:00Z","timestamp":1498176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R00\u2010DC\u2010011322"],"award-info":[{"award-number":["R00\u2010DC\u2010011322"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    (pneumococcus) is a major human pathogen. It is a common colonizer of the human respiratory track, where it utilizes cell\u2013cell communication systems to coordinate population\u2010level behaviors. We reasoned that secreted peptides that are highly expressed during infection are pivotal for virulence. Thus, we used\n                    <jats:italic>in silico<\/jats:italic>\n                    pattern searches to define a pneumococcal secretome and analyzed the transcriptome of the clinically important PMEN1 lineage to identify which peptide\u2010encoding genes are highly expressed\n                    <jats:italic>in vivo<\/jats:italic>\n                    . In this study, we characterized virulence peptide 1 (\n                    <jats:italic>vp1<\/jats:italic>\n                    ), a highly expressed Gly\u2013Gly peptide\u2010encoding gene in chinchilla middle ear effusions. The\n                    <jats:italic>vp1<\/jats:italic>\n                    gene is widely distributed across pneumococcus as well as encoded in related species. Studies in the chinchilla model of middle ear infection demonstrated that VP1 is a virulence determinant. The\n                    <jats:italic>vp1<\/jats:italic>\n                    gene is positively regulated by a transcription factor from the Rgg family and its cognate SHP (short hydrophobic peptide).\n                    <jats:italic>In vitro<\/jats:italic>\n                    data indicated that VP1 promotes increased thickness and biomass for biofilms grown on chinchilla middle ear epithelial cells. Furthermore, the wild\u2010type biofilm is restored with the exogenous addition of synthetic VP1. We conclude that VP1 is a novel streptococcal regulatory peptide that controls biofilm development and pneumococcal pathogenesis.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13721","type":"journal-article","created":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T20:02:50Z","timestamp":1496088170000},"page":"554-571","source":"Crossref","is-referenced-by-count":42,"title":["A novel streptococcal cell\u2013cell communication peptide promotes pneumococcal virulence and biofilm formation"],"prefix":"10.1111","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3571-5845","authenticated-orcid":false,"given":"Rolando A.","family":"Cuevas","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rory","family":"Eutsey","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anagha","family":"Kadam","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob A.","family":"West\u2010Roberts","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol A.","family":"Woolford","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Mason","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis The Research Institute at Nationwide Children's Hospital Columbus OH 43205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. Luisa","family":"Hiller","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Carnegie Mellon University Pittsburgh PA 15213, USA"},{"name":"Center of Excellence in Biofilm Research, Allegheny Health Network Pittsburgh PA 15211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00393-15"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.583989"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43587"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004076"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp335"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00749-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066042"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.028282-0"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn149"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001220"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026707"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058492"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0168461"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13088"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002190"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00194"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00194"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.02.009"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.60.080805.142139"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1613"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574-6976.12046"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00382-13"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.42.100188.000525"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2625"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002745"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1198545"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.12.3928-3937.2004"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01775-05"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.17.4696-4703.2000"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502349200"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r107"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01723.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00173-15"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044250"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20195"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07633.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074867"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1385"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500879102"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.296.2.202"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.24.11140"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02295.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00917-07"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01004-07"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028850"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1177\/000348940911800410"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13029"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.4161\/mge.25582"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006339"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc09d04s36"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1871"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00520-13"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01490-14"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02920-12"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00266-8"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00333-12.Editor"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw290"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/s120302519"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00474-07"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01656-15"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00438-13"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00488-12"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1086\/514242"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-14-745"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-7-2045"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5582"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1226"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121042"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.1.1-34.1998"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.501417.x"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldn030"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e3282b97327"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.20.6087-6090.1996"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00942-12"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09291.x"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2651-y"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01303-13"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.11.040"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-245"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000221954.05467.54"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05321-1"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00721-09"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01725.x"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-75"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw256"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6411"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05186-11"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"e_1_2_7_91_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis703"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13721","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13721","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/mmi.13721","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13721","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T07:04:13Z","timestamp":1694156653000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13721"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,23]]},"references-count":90,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1111\/mmi.13721"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13721","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,23]]}}}