{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:10:28Z","timestamp":1790226628256,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,7,12]],"date-time":"2017-07-12T00:00:00Z","timestamp":1499817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research","doi-asserted-by":"publisher","award":["BSC0104"],"award-info":[{"award-number":["BSC0104"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Peptidoglycan (PG) is an essential, envelope\u2010fortifying macromolecule of eubacterial cell walls. It is a large polymer with multiple glycan strands interconnected by short peptide chains forming a sac\u2010like structure around cytoplasmic membrane. In most bacteria, the composition of the peptide chain is well\u2010conserved and distinctive; in\n                    <jats:italic>E. coli<\/jats:italic>\n                    , the peptide chain length varies from two to five amino acids with a tetrapeptide consisting of L\u2010alanine \u2013 D\u2010glutamic acid \u2013\n                    <jats:italic>meso<\/jats:italic>\n                    \u2010diaminopimelic acid \u2013 D\u2010alanine. However, it is not known how bacteria conserve the composition and sequence of peptide chains of PG. Here, we find that a conserved open reading frame of unknown function, YfiH (renamed PgeF) contributes to the maintenance of peptide composition in\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Using genetic, biochemical and mass spectrometrical analyses we demonstrate that absence of\n                    <jats:italic>yfiH<\/jats:italic>\n                    results in incorporation of non\u2010canonical amino acids, L\u2010serine or glycine in place of L\u2010alanine in PG sacculi leading to \u03b2\u2010lactam \u2013 sensitivity, lethality in mutants defective in PG remodelling or recycling pathways, altered cell morphology and reduced PG synthesis.\n                    <jats:italic>yfiH<\/jats:italic>\n                    orthologs from other Gram\u2010positive genera were able to compensate the absence of\n                    <jats:italic>yfiH<\/jats:italic>\n                    in\n                    <jats:italic>E. coli<\/jats:italic>\n                    indicating a conserved pathway in bacterial kingdom. Our results suggest editing\/quality control mechanisms exist to maintain composition and integrity of bacterial peptidoglycan.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13730","type":"journal-article","created":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T08:02:48Z","timestamp":1497427368000},"page":"705-720","source":"Crossref","is-referenced-by-count":24,"title":["Identification of YfiH (PgeF) as a factor contributing to the maintenance of bacterial peptidoglycan composition"],"prefix":"10.1111","volume":"105","author":[{"given":"Sadiya","family":"Parveen","sequence":"first","affiliation":[{"name":"CSIR\u2010Centre for Cellular and Molecular Biology Hyderabad 500007 Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0387-0856","authenticated-orcid":false,"given":"Manjula","family":"Reddy","sequence":"additional","affiliation":[{"name":"CSIR\u2010Centre for Cellular and Molecular Biology Hyderabad 500007 Telangana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.175"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2008.00104.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600577200"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00089.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3532"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.246"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.23.6434-6436.2002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(88)90468-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81481-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1656(94)90131-7"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.3.906-910.1996"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(75)90324-3"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.33.1577"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.1.181-203.1998"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20589"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1991.tb04645.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.0080i.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00906-09"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3212"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.23.6827-6830.2000"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3529391"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(66)90221-X"},{"key":"e_1_2_7_27_1","volume-title":"A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia Coli and Related Bacteria","author":"Miller J.H.","year":"1992"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.11.052"},{"key":"e_1_2_7_29_1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1128\/jb.151.2.777-782.1982","article-title":"L\u2010Serine degradation in Escherichia coli K\u201012: A combination of L\u2010serine, glycine, and leucine used as a source of carbon","volume":"151","author":"Newman E.B.","year":"1982","journal-title":"J Bacteriol"},{"key":"e_1_2_7_30_1","first-page":"2P","article-title":"Inhibition of cell wall synthesis in Staphylococcus aureus by chemicals which cause accumulation of wall precursors","volume":"70","author":"Park J.T.","year":"1958","journal-title":"Biochem J"},{"key":"e_1_2_7_31_1","first-page":"48","volume-title":"Escherichia Coli and Salmonella: Cellular and Molecular Biology","author":"Park J.T.","year":"1996"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00027-07"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2010.07.009"},{"key":"e_1_2_7_34_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2008.00105.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/br.36.4.407-477.1972"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb18938.x"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12058"},{"key":"e_1_2_7_39_1","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1128\/jb.89.4.1124-1127.1965","article-title":"Nucleotide accumulation induced in Staphylococcus aureus by Glycine","volume":"89","author":"Strominger J.L.","year":"1965","journal-title":"J Bacteriol"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2677"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00446-07"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.261348198"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2007.00094.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38877"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.164.1.331-337.1985"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2008.06315.x"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00748-10"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13730","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T12:03:23Z","timestamp":1696248203000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,12]]},"references-count":46,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/mmi.13730"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13730","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727712550.793537510","asserted-by":"object"}]},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,12]]}}}