{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:53:05Z","timestamp":1790599985070,"version":"4.1.0"},"reference-count":91,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T00:00:00Z","timestamp":1501804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI028798 and AI110325"],"award-info":[{"award-number":["AI028798 and AI110325"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The infectious metacyclic forms of\n                    <jats:italic>Trypanosoma brucei<\/jats:italic>\n                    result from a complex development in the tsetse fly vector. When they infect mammals, they cause African sleeping sickness in humans. Due to scarcity of biological material and difficulties of the tsetse fly as an experimental system, very limited information is available concerning the gene expression profile of metacyclic forms. We used an\n                    <jats:italic>in vitro<\/jats:italic>\n                    system based on expressing the RNA binding protein 6 to obtain infectious metacyclics and determined their protein and mRNA repertoires by mass\u2010spectrometry (MS) based proteomics and mRNA sequencing (RNA\u2010Seq) in comparison to non\u2010infectious procyclic trypanosomes. We showed that metacyclics are quiescent cells, and propose this influences the choice of a monocistronic variant surface glycoprotein expression site. Metacyclics have a largely bloodstream\u2010form type transcriptome, and thus are programmed to translate a bloodstream\u2010form type proteome upon entry into the mammalian host and resumption of cell division. Genes encoding cell surface components showed the largest changes between procyclics and metacyclics, observed at both the transcript and protein levels. Genes encoding metabolic enzymes exhibited expression in metacyclics with features of both procyclic and bloodstream forms, suggesting that this intermediate\u2010type metabolism is dictated by the availability of nutrients in the tsetse fly vector.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13754","type":"journal-article","created":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T10:28:39Z","timestamp":1500978519000},"page":"74-92","source":"Crossref","is-referenced-by-count":63,"title":["The proteome and transcriptome of the infectious metacyclic form of\n                    <i>Trypanosoma brucei<\/i>\n                    define quiescent cells primed for mammalian invasion"],"prefix":"10.1111","volume":"106","author":[{"given":"Romain","family":"Christiano","sequence":"first","affiliation":[{"name":"Department of Cell Biology, School of Medicine Yale University 333 Cedar Street, New Haven, CT 06520 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolay G.","family":"Kolev","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Microbial Diseases Yale School of Public Health 60 College Street, New Haven, CT 06520 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huafang","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Microbial Diseases Yale School of Public Health 60 College Street, New Haven, CT 06520 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Ullu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine Yale University 333 Cedar Street, New Haven, CT 06520 USA"},{"name":"Department of Internal Medicine, School of Medicine Yale University 330 Cedar St, Boardman 110, New Haven, CT 06520 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias C.","family":"Walther","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, School of Medicine Yale University 333 Cedar Street, New Haven, CT 06520 USA"},{"name":"Howard Hughes Medical Institute Harvard T.H. Chan School of Public Health Boston  MA 02115 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9365-5254","authenticated-orcid":false,"given":"Christian","family":"Tschudi","sequence":"additional","affiliation":[{"name":"Department of Epidemiology of Microbial Diseases Yale School of Public Health 60 College Street, New Haven, CT 06520 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