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Here, we show that the introduction of a single lipid\u2010facing arginine residue near the middle of the \u03b2 barrel of the <jats:italic>Escherichia coli<\/jats:italic> OMPs OmpLA and EspP creates an energy barrier that impedes membrane insertion. Although several unintegrated OmpLA mutants remained insertion\u2010competent, they were slowly degraded by the periplasmic protease DegP. Two EspP mutants were also gradually degraded by DegP but were toxic because they first bound to the Bam complex, an essential heteroligomer that catalyzes the membrane insertion of OMPs. Interestingly, another EspP mutant likewise formed a prolonged, deleterious interaction with the Bam complex but was protected from degradation and eventually inserted into the membrane in a native conformation. The different types of interactions between the EspP mutants and the Bam complex that we observed may correspond to distinct stages in OMP assembly. Our results show that sequences that significantly delay assembly are disfavored not only because unintegrated OMPs are subjected to degradation, but also because OMPs that assemble slowly can form dominant\u2010negative interactions with the Bam complex.<\/jats:p>","DOI":"10.1111\/mmi.13845","type":"journal-article","created":{"date-parts":[[2017,9,23]],"date-time":"2017-09-23T09:42:48Z","timestamp":1506159768000},"page":"777-792","source":"Crossref","is-referenced-by-count":20,"title":["Selective pressure for rapid membrane integration constrains the sequence of bacterial outer membrane proteins"],"prefix":"10.1111","volume":"106","author":[{"given":"Janine H.","family":"Peterson","sequence":"first","affiliation":[{"name":"Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashlee M.","family":"Plummer","sequence":"additional","affiliation":[{"name":"Thomas C. Jenkins Department of Biophysics Johns Hopkins University, 3400 North Charles Street Baltimore MD 21218 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen G.","family":"Fleming","sequence":"additional","affiliation":[{"name":"Thomas C. Jenkins Department of Biophysics Johns Hopkins University, 3400 North Charles Street Baltimore MD 21218 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4941-3741","authenticated-orcid":false,"given":"Harris D.","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Genetics and Biochemistry Branch National Institute of Diabetes and Digestive and Kidney Diseases National Institutes of Health Bethesda MD 20892 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