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However, the biotransformation mechanisms of indole are still poorly studied. Here, we sought to unlock the genetic determinants of indole biotransformation in strain <jats:italic>Cupriavidus<\/jats:italic> sp. SHE based on genomics, proteomics and functional studies. A total of 177 proteins were notably altered (118 up\u2010 and 59 downregulated) in cells grown in indole mineral salt medium when compared with that in sodium citrate medium. RT\u2010qPCR and gene knockout assays demonstrated that an indole oxygenase gene cluster was responsible for the indole upstream metabolism. A functional indole oxygenase, termed IndA, was identified in the cluster, and its catalytic efficiency was higher than those of previously reported indole oxidation enzymes. Furthermore, the indole downstream metabolism was found to proceed via the atypical CoA\u2010thioester pathway rather than conventional gentisate and salicylate pathways. This unusual pathway was catalyzed by a conserved 2\u2010aminobenzoyl\u2010CoA gene cluster, among which the 2\u2010aminobenzoyl\u2010CoA ligase initiated anthranilate transformation. This study unveils the genetic determinants of indole biotransformation and will provide new insights into our understanding of indole biodegradation in natural environments and its functional studies.<\/jats:p>","DOI":"10.1111\/mmi.13852","type":"journal-article","created":{"date-parts":[[2017,9,30]],"date-time":"2017-09-30T08:22:37Z","timestamp":1506759757000},"page":"905-918","source":"Crossref","is-referenced-by-count":51,"title":["Unveiling the biotransformation mechanism of indole in a <i>Cupriavidus<\/i> sp. strain"],"prefix":"10.1111","volume":"106","author":[{"given":"Yuanyuan","family":"Qu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology Dalian University of Technology  Dalian 116024 People's Republic of 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