{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:21:17Z","timestamp":1790666477736,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T00:00:00Z","timestamp":1522972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM037219"],"award-info":[{"award-number":["GM037219"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Transcription and translation are coupled processes in bacteria. A role of transcription elongation cofactor NusG in coupling has been suggested by\n                    <jats:italic>in vitro<\/jats:italic>\n                    structural studies. NMR revealed association of the NusG carboxy\u2010terminal domain with S10 (NusE), implying a direct role for NusG as a bridge linking RNAP and the lead ribosome. Here we present the first\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    evidence of full\u2010length NusG association with mature 70S ribosomes. Binding did not require accessory factors\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Mutating the NusG:S10 binding interface at NusG F165 or NusE M88 and D97 residues weakened NusG:S10 association\n                    <jats:italic>in vivo<\/jats:italic>\n                    and completely abolished it\n                    <jats:italic>in vitro<\/jats:italic>\n                    , supporting the specificity of this interaction. Mutations in the binding interface increased sensitivity to chloramphenicol. This phenotype was suppressed by\n                    <jats:italic>rpoB<\/jats:italic>\n                    *35, an RNAP mutation that reduces replisome\u2010RNAP clashes. We propose that weakened NusG:S10 interaction leads to uncoupling when translation is inhibited, with resulting RNAP backtracking, replication blocks and formation of lethal DNA double\u2010strand breaks.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13953","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T12:14:29Z","timestamp":1521980069000},"page":"495-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":81,"title":["<i>Escherichia coli<\/i>\n                    transcription factor NusG binds to 70S ribosomes"],"prefix":"10.1111","volume":"108","author":[{"given":"Shivalika","family":"Saxena","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Columbia University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6122-2817","authenticated-orcid":false,"given":"Kamila K.","family":"Myka","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Columbia University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Washburn","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Columbia University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Costantino","sequence":"additional","affiliation":[{"name":"Gene Regulation and Chromosome Biology Laboratory National Cancer Institute Frederick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald L.","family":"Court","sequence":"additional","affiliation":[{"name":"Gene Regulation and Chromosome Biology Laboratory National Cancer Institute Frederick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max E.","family":"Gottesman","sequence":"additional","affiliation":[{"name":"Department of 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