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In our previous work, we reconstructed the metabolic models of this species along with two other mycoplasmas from the respiratory tract of swine:<jats:italic>Mycoplasma hyorhinis<\/jats:italic>, considered less pathogenic but which nonetheless causes disease and<jats:italic>Mycoplasma flocculare<\/jats:italic>, a commensal bacterium. We identified metabolic differences that partially explained their different levels of pathogenicity. One important trait was the production of hydrogen peroxide from the glycerol metabolism only in the pathogenic species. Another important feature was a pathway for the metabolism of myo\u2010inositol in<jats:italic>M. hyopneumoniae<\/jats:italic>. Here, we tested these traits to understand their relation to the different levels of pathogenicity, comparing not only the species but also pathogenic and attenuated strains of<jats:italic>M. hyopneumoniae<\/jats:italic>. Regarding the myo\u2010inositol metabolism, we show that only<jats:italic>M. hyopneumoniae<\/jats:italic>assimilated this carbohydrate and remained viable when myo\u2010inositol was the primary energy source. Strikingly, only the two pathogenic strains of<jats:italic>M. hyopneumoniae<\/jats:italic>produced hydrogen peroxide in complex medium. We also show that this production was dependent on the presence of glycerol. Although further functional tests are needed, we present in this work two interesting metabolic traits of<jats:italic>M. hyopneumoniae<\/jats:italic>that might be directly related to its enhanced virulence.<\/jats:p>","DOI":"10.1111\/mmi.13957","type":"journal-article","created":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T22:33:50Z","timestamp":1523054030000},"page":"683-696","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Hydrogen peroxide production and myo\u2010inositol metabolism as important traits for virulence of<i>Mycoplasma hyopneumoniae<\/i>"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9574-9991","authenticated-orcid":false,"given":"Mariana Galv\u00e3o","family":"Ferrarini","sequence":"first","affiliation":[{"name":"ERABLE Team Institut Nationale de Recherche en Informatique et Automation Villeurbanne France"},{"name":"Laboratoire de Biometrie et Biologie Evolutive Universite Claude Bernard Lyon 1 Villeurbanne France"},{"name":"Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scheila Gabriele","family":"Mucha","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Parrot","sequence":"additional","affiliation":[{"name":"ERABLE Team Institut Nationale de Recherche en Informatique et Automation Villeurbanne France"},{"name":"Laboratoire de Biometrie et Biologie Evolutive Universite Claude Bernard Lyon 1 Villeurbanne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Meiffrein","sequence":"additional","affiliation":[{"name":"Centre d'Etude des Substances Naturelles Universite Claude Bernard Lyon 1 Villeurbanne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Fernando","family":"Ruggiero Bachega","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"},{"name":"Departamento de Farmacociencias Universidade Federal de Ciencias da Saude de Porto Alegre Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Comte","sequence":"additional","affiliation":[{"name":"Centre d'Etude des Substances Naturelles Universite Claude Bernard Lyon 1 Villeurbanne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaldo","family":"Zaha","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia Universidade Federal do Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010France","family":"Sagot","sequence":"additional","affiliation":[{"name":"ERABLE Team Institut 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