{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:16:57Z","timestamp":1740125817792,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T00:00:00Z","timestamp":1524700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS095063","NS102337"],"award-info":[{"award-number":["NS095063","NS102337"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Most fungi express two transcription factors that regulate the expression of genes associated with copper uptake for nutritional needs, and with copper resistance when copper approaches a cytotoxic level. These factors are characterized by cysteine\u2010rich motifs which are associated with copper\u2010sensing, DNA\u2010binding and release, and\/or cytoplasmic retention. <jats:italic>Cryptococcus neoformans<\/jats:italic> differs from most in that it expresses a single such copper\u2010sensing <jats:italic>trans<\/jats:italic>\u2010factor, Cuf1, a protein that up\u2010regulates copper uptake when copper is scarce, and up\u2010regulates copper sequestration when cells become super\u2010replete. For <jats:italic>C. neoformans<\/jats:italic> this is an essential task in as much as copper is relatively bioavailable in lung airways while the brain interstitium can be copper\u2010limiting for growth. While fungal dependence on and sensitivity to copper have long been considered targets for anti\u2010fungal chemistry, fungi have proven adept at finding \u2018work arounds\u2019 by using a chelated form of copper as nutrient or adapting to a copper\u2010surfaced hospital bed by increased resistance. However, the cohort of Cuf1 targets identified in this report represent far more than just the uptake and sequestration machinery, but include additional loci that, perhaps, are less easily \u2018defended\u2019 by the fungus. Garcia\u2010Santamarina <jats:italic>et al<\/jats:italic>. provide that list and thus lay the ground\u2010work for developing novel anti\u2010fungal reagents.<\/jats:p>","DOI":"10.1111\/mmi.13963","type":"journal-article","created":{"date-parts":[[2018,4,10]],"date-time":"2018-04-10T06:45:25Z","timestamp":1523342725000},"page":"463-466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["For <i>Cryptococcus neoformans<\/i>, responding to the copper status in a colonization niche is not just about copper"],"prefix":"10.1111","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3570-7682","authenticated-orcid":false,"given":"Daniel J.","family":"Kosman","sequence":"first","affiliation":[{"name":"Department of Biochemistry, The Jacobs School of Medicine and Biomedical Sciences The University at Buffalo Buffalo NY 14203 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,26]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.05.013"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.2.277-292.2006"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07794.x"},{"key":"e_1_2_3_5_1","first-page":"80","article-title":"Historic uses of copper\u2010compounds in medicine","volume":"2","author":"Dollwet H.H.A.","year":"1985","journal-title":"Trace Elem Med"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.17.5342"},{"key":"e_1_2_3_7_1","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1111\/mmi.13960","article-title":"Genome\u2010wide analysis of the regulation of Cu metabolism in Cryptococcus neoformans","volume":"108","author":"Garcia\u2010Santamaria S.","year":"2018","journal-title":"Mol Microbiol."},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02766-10"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6MT00172F"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2836"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2011.00733.x"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/acprof:oso\/9780195189711.001.0001"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.17.4899-4905.2000"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.01.009"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6550"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04474.x"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3520"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fmmi.13963","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/mmi.13963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:48:57Z","timestamp":1695494937000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/mmi.13963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,26]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1111\/mmi.13963"],"URL":"https:\/\/doi.org\/10.1111\/mmi.13963","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"type":"print","value":"0950-382X"},{"type":"electronic","value":"1365-2958"}],"subject":[],"published":{"date-parts":[[2018,4,26]]},"assertion":[{"value":"2018-03-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}