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Here we characterize SOPT, a protein expressed in\n                    <jats:italic>P. falciparum<\/jats:italic>\n                    ookinetes that facilitates infection of the mosquito midgut. SOPT was identified on the basis that it contains a signal peptide, a PEXEL\u2010like sequence and is expressed in asexual, ookinete and sporozoite stages, suggesting it is involved in infecting the human or mosquito host. SOPT is predicted to contain a subtilisin\u2010like fold with a non\u2010canonical catalytic triad and is orthologous to\n                    <jats:italic>P.\u00a0berghei<\/jats:italic>\n                    PIMMS2. Localization studies reveal that SOPT is not exported to the erythrocyte but is expressed in ookinetes at the parasite periphery.\n                    <jats:italic>SOPT<\/jats:italic>\n                    \u2010deficient parasites develop normally through the asexual and sexual stages and produce equivalent numbers of ookinetes to NF54 controls, however, they form fewer oocysts and sporozoites in mosquitoes. SOPT\u2010deficient parasites were also unable to activate the immune\u2010responsive midgut invasion marker\n                    <jats:italic>SRPN6<\/jats:italic>\n                    after mosquito ingestion, suggesting they are defective for entry into the midgut. Disruption of\n                    <jats:italic>SOPT<\/jats:italic>\n                    in\n                    <jats:italic>P. berghei<\/jats:italic>\n                    (PIMMS2) did not affect other lifecycle stages or ookinete development but again resulted in fewer oocysts and sporozoites in mosquitoes. Collectively, this study shows that SOPT\/PIMMS2 plays a conserved role in ookinetes of different\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species.\n                  <\/jats:p>","DOI":"10.1111\/mmi.13993","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T01:36:55Z","timestamp":1528249015000},"page":"458-473","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Plasmodium falciparum<\/i>\n                    subtilisin\u2010like ookinete protein SOPT plays an important and conserved role during ookinete infection of the\n                    <i>Anopheles stephensi<\/i>\n                    midgut"],"prefix":"10.1111","volume":"109","author":[{"given":"Jennifer S.","family":"Armistead","sequence":"first","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research Parkville 3052 Australia"},{"name":"Department of Medical Biology The University of Melbourne Parkville 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlie","family":"Jennison","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research Parkville 3052 Australia"},{"name":"Department of Medical Biology The University of Melbourne Parkville 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"O'Neill","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research Parkville 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sash","family":"Lopaticki","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research Parkville 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Liehl","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa 1649\u2010028 Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten K.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Molecular, Faculdade de Medicina Universidade de Lisboa 1649\u2010028 Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Annoura","sequence":"additional","affiliation":[{"name":"Leiden Malaria Research Group, Parasitology Leiden University Medical Centre 2333ZA Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pravin","family":"Rajasekaran","sequence":"additional","affiliation":[{"name":"The Walter and Eliza Hall Institute of Medical Research Parkville 3052 Australia"},{"name":"Department of Medical Biology The University of Melbourne Parkville 3052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara 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