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However, little is known about the composition or function of the flagellar collar proteins. To identify a collar protein, we have inactivated almost all genes annotated as motility\u2010related in the <jats:italic>Borrelia burgdorferi<\/jats:italic> genome and identified only FlbB, which comprises the base of the collar. Since the major components of the collar complex remained unidentified, we took advantage of a protein\u2013protein interaction map developed in another\u00a0spirochete,\u00a0<jats:italic>Treponema pallidum<\/jats:italic> to identify proteins of unknown function that could be collar proteins. Subsequently, using various comprehensive approaches, we identified a tetratricopeptide repeat protein BB0236 as a potential candidate for the collar. Biochemical assays indicated that FlbB interacts with BB0236. Furthermore, \u2206<jats:italic>bb0236<\/jats:italic> mutant analyses indicated that BB0236 is crucial for collar structure assembly, cellular morphology, motility, orientation of periplasmic flagella and assembly of other flagellar structures. Moreover, using comparative motor analyses, we propose how the collar structure is assembled in <jats:italic>B. burgdorferi<\/jats:italic>. Together, our studies provide new insights into the organization and the complex assembly inherent to the unique spirochetal collar structure.<\/jats:p>","DOI":"10.1111\/mmi.14121","type":"journal-article","created":{"date-parts":[[2018,10,10]],"date-time":"2018-10-10T17:21:34Z","timestamp":1539192094000},"page":"634-647","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["A tetratricopeptide repeat domain protein has profound effects on assembly of periplasmic flagella, morphology and motility of the lyme disease spirochete <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1111","volume":"110","author":[{"given":"Ki Hwan","family":"Moon","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Brody School of Medicine, East Carolina University Greenville NC 27834 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