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Calcium\u2010dependent protein kinase 3 (<jats:italic>Tg<\/jats:italic>CDPK3) was previously identified as a key mediator in this process, as <jats:italic>Tg<\/jats:italic>CDPK3 knockout (\u2206<jats:italic>cdpk3<\/jats:italic>) parasites fail to egress in a timely manner. Phosphoproteomic analysis comparing WT with \u2206<jats:italic>cdpk3<\/jats:italic> parasites revealed changes in the <jats:italic>Tg<\/jats:italic>CDPK3\u2010dependent phosphoproteome that included proteins important for regulating motility, but also metabolic enzymes, indicating that <jats:italic>Tg<\/jats:italic>CDPK3 controls processes beyond egress. Here we have investigated a predicted direct target of <jats:italic>Tg<\/jats:italic>CDPK3, ApiAT5\u20103, a putative transporter of the major facilitator superfamily, and show that it is rapidly phosphorylated at serine 56 after induction of calcium signalling. Conditional knockout of <jats:italic>apiAT5\u20103<\/jats:italic> results in transcriptional upregulation of most ribosomal subunits, but no alternative transporters, and subsequent parasite death. Mutating the S56 to a non\u2010phosphorylatable alanine leads to a fitness cost, suggesting that phosphorylation of this residue is beneficial, albeit not essential, for tyrosine import. Using a combination of metabolomics and heterologous expression, we confirmed a primary role in tyrosine import for ApiAT5\u20103. However, no significant differences in tyrosine import could be detected in phosphorylation site mutants showing that if tyrosine transport is affected by S56 phosphorylation, its regulatory role is subtle.<\/jats:p>","DOI":"10.1111\/mmi.14156","type":"journal-article","created":{"date-parts":[[2018,11,7]],"date-time":"2018-11-07T02:25:37Z","timestamp":1541557537000},"page":"1167-1181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Characterisation of the <i>Toxoplasma gondii<\/i> tyrosine transporter and its phosphorylation by the calcium\u2010dependent protein kinase 3"],"prefix":"10.1111","volume":"111","author":[{"given":"Bethan A.","family":"Wallbank","sequence":"first","affiliation":[{"name":"Signalling in Apicomplexan Parasites Laboratory The Francis Crick Institute London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caia 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