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In this study, we have defined a role for a conserved gene of unknown function in\n                    <jats:italic>B. abortus<\/jats:italic>\n                    envelope stress resistance and infection. Expression of this gene, which we name\n                    <jats:italic>eipA<\/jats:italic>\n                    , is directly activated by the essential cell cycle regulator, CtrA.\n                    <jats:italic>eipA<\/jats:italic>\n                    encodes a soluble periplasmic protein that adopts an unusual eight\u2010stranded \u03b2\u2010barrel fold. Deletion of\n                    <jats:italic>eipA<\/jats:italic>\n                    attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O\u2010polysaccharide biosynthesis is synthetically lethal with\n                    <jats:italic>eipA<\/jats:italic>\n                    deletion. This genetic connection between O\u2010polysaccharide and\n                    <jats:italic>eipA<\/jats:italic>\n                    is corroborated by our discovery that\n                    <jats:italic>eipA<\/jats:italic>\n                    is essential in\n                    <jats:italic>Brucella ovis<\/jats:italic>\n                    , a naturally rough species that harbors mutations in several genes required for O\u2010polysaccharide production. Conditional depletion of\n                    <jats:italic>eipA<\/jats:italic>\n                    expression in\n                    <jats:italic>B. ovis<\/jats:italic>\n                    results in a cell chaining phenotype, providing evidence that\n                    <jats:italic>eipA<\/jats:italic>\n                    directly or indirectly influences cell division in\n                    <jats:italic>Brucella<\/jats:italic>\n                    . We conclude that EipA is a molecular determinant of\n                    <jats:italic>Brucella<\/jats:italic>\n                    virulence that functions to maintain cell envelope integrity and influences cell division.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14178","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T07:55:08Z","timestamp":1544255708000},"page":"637-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Periplasmic protein EipA determines envelope stress resistance and virulence in\n                    <i>Brucella abortus<\/i>"],"prefix":"10.1111","volume":"111","author":[{"given":"Julien","family":"Herrou","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology The University of Chicago Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan 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