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Previous studies determined that the <jats:italic>B. burgdorferi<\/jats:italic> BpuR protein binds to its own mRNA and autoregulates its translation, and also serves as co\u2010repressor of <jats:italic>erp<\/jats:italic> transcription. Here, we demonstrate that <jats:italic>B. burgdorferi<\/jats:italic> controls transcription of <jats:italic>bpuR<\/jats:italic>, expressing high levels of <jats:italic>bpuR<\/jats:italic> during tick colonization but significantly less during mammalian infection. The master regulator of chromosomal replication, DnaA, was found to bind specifically to a DNA sequence that overlaps the <jats:italic>bpuR<\/jats:italic> promoter. Cultured <jats:italic>B. burgdorferi<\/jats:italic> that were genetically manipulated to produce elevated levels of BpuR exhibited altered levels of several proteins, although BpuR did not impact mRNA levels. Among these was the SodA superoxide dismutase, which is essential for mammalian infection. BpuR bound to <jats:italic>sodA<\/jats:italic> mRNA in live <jats:italic>B. burgdorferi<\/jats:italic>, and a specific BpuR\u2010binding site was mapped 5\u2032 of the <jats:italic>sodA<\/jats:italic> open reading frame. Recognition of posttranscriptional regulation of protein levels by BpuR adds another layer to our understanding of the <jats:italic>B. burgdorferi<\/jats:italic> regulome, and provides further evidence that bacterial protein levels do not always correlate directly with mRNA levels.<\/jats:p>","DOI":"10.1111\/mmi.14336","type":"journal-article","created":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T01:23:55Z","timestamp":1561512235000},"page":"973-991","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The Lyme disease spirochete's BpuR DNA\/RNA\u2010binding protein is differentially expressed during the mammal\u2013tick infectious cycle, which affects translation of the SodA superoxide dismutase"],"prefix":"10.1111","volume":"112","author":[{"given":"Brandon L.","family":"Jutras","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Molecular Genetics University of Kentucky 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