{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:09:21Z","timestamp":1790834961801,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,8,18]],"date-time":"2019-08-18T00:00:00Z","timestamp":1566086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Salmonella enterica<\/jats:italic> serovar Typhimurium is a facultative intracellular pathogen that invades the intestinal epithelium. Following invasion of epithelial cells, <jats:italic>Salmonella<\/jats:italic> survives and replicates within two distinct intracellular niches. While all of the bacteria are initially taken up into a membrane bound vacuole, the <jats:italic>Salmonella<\/jats:italic>\u2010containing vacuole or SCV, a significant proportion of them promptly escape into the cytosol. Cytosolic <jats:italic>Salmonella<\/jats:italic> replicates more rapidly compared to the vacuolar population, although the reasons for this are not well understood. SipA, a multi\u2010function effector protein, has been shown to affect intracellular replication and is secreted by cytosolic <jats:italic>Salmonella<\/jats:italic> via the invasion\u2010associated Type III Secretion System 1 (T3SS1). Here, we have used a multipronged microscopy approach to show that SipA does not affect bacterial replication rates <jats:italic>per se<\/jats:italic>, but rather mediates intra\u2010cytosolic survival and\/or initiation of replication following bacterial egress from the SCV. Altogether, our findings reveal an important role for SipA in the early survival of cytosolic <jats:italic>Salmonella<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/mmi.14361","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T22:08:16Z","timestamp":1564697296000},"page":"1270-1283","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["A role for the <i>Salmonella<\/i> Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells"],"prefix":"10.1111","volume":"112","author":[{"given":"Audrey","family":"Chong","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institutes of Allergy and Infectious Diseases National Institutes of Health Hamilton MT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tregei","family":"Starr","sequence":"additional","affiliation":[{"name":"Laboratory of 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