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The SPI4\u2010encoded T1SS consists of five proteins (SiiABCDF) and secretes the giant adhesin SiiE. Here, we investigated structure\u2013function relationships in SiiA, a non\u2010canonical T1SS subunit. We show that SiiA consists of a membrane domain, an intrinsically disordered periplasmic linker region and a folded globular periplasmic domain (SiiA\u2010PD). The crystal structure of SiiA\u2010PD displays homology to that of MotB and other peptidoglycan (PG)\u2010binding domains. SiiA\u2010PD binds PG\n                    <jats:italic>in vitro<\/jats:italic>\n                    , albeit at an acidic pH, only. Mutation of Arg162 impedes PG binding of SiiA and reduces\n                    <jats:italic>Salmonella<\/jats:italic>\n                    invasion efficacy. SiiA forms a complex with SiiB at the inner membrane (IM), and the observed SiiA\u2010MotB homology is paralleled by a predicted SiiB\u2010MotA homology. We show that, similar to MotAB, SiiAB translocates protons across the IM. Mutating Asp13 in SiiA impairs proton translocation. Overall, SiiA shares numerous properties with MotB. However, MotAB uses the proton motif force (PMF) to energize the bacterial flagellum, it remains to be shown how usage of the PMF by SiiAB assists T1SS function and\n                    <jats:italic>Salmonella<\/jats:italic>\n                    invasion.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14368","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T14:50:26Z","timestamp":1565967026000},"page":"1403-1422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Structural and functional characterization of SiiA, an auxiliary protein from the SPI4\u2010encoded type 1 secretion system from\n                    <i>Salmonella enterica<\/i>"],"prefix":"10.1111","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9162-9485","authenticated-orcid":false,"given":"Peter","family":"Kirchweger","sequence":"first","affiliation":[{"name":"Division of Biotechnology, Department of Biology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg Henkestr. 91 D\u201091052 Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigrid","family":"Weiler","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, Department of Biology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg Henkestr. 91 D\u201091052 Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Egerer\u2010Sieber","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, Department of Biology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg Henkestr. 91 D\u201091052 Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna\u2010Theresa","family":"Blasl","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, Department of Biology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg Henkestr. 91 D\u201091052 Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Robert Koch\u2010Institut Wernigerode Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Robert Koch\u2010Institut Wernigerode Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Sander","sequence":"additional","affiliation":[{"name":"Abt. Mikrobiologie and CellNanOs Universit\u00e4t Osnabr\u00fcck Osnabr\u00fcck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothee","family":"Merker","sequence":"additional","affiliation":[{"name":"Abt. Mikrobiologie and CellNanOs Universit\u00e4t Osnabr\u00fcck Osnabr\u00fcck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5718-4758","authenticated-orcid":false,"given":"Roman G.","family":"Gerlach","sequence":"additional","affiliation":[{"name":"Robert Koch\u2010Institut Wernigerode Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Hensel","sequence":"additional","affiliation":[{"name":"Abt. Mikrobiologie and CellNanOs Universit\u00e4t Osnabr\u00fcck Osnabr\u00fcck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0519-8928","authenticated-orcid":false,"given":"Yves A.","family":"Muller","sequence":"additional","affiliation":[{"name":"Division of Biotechnology, Department of Biology Friedrich\u2010Alexander\u2010University Erlangen\u2010N\u00fcrnberg Henkestr. 91 D\u201091052 Erlangen 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