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However, only a small number of molecular complexes involved in trafficking, tethering and fusion of vesicles have been identified in\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    . Consequently, it is unclear how complicated vesicle trafficking is accomplished in this parasite. Sec1\/Munc18\u2010like (SM) proteins are essential components of protein complexes involved in vesicle fusion. Here, we found that depletion of the SM protein TgSec1 using an auxin\u2010inducible degron\u2010based conditional knockout strategy led to mislocalization of plasma membrane proteins. By contrast, conditional depletion of the SM protein TgVps45 led to morphological changes, asymmetrical loss of the inner membrane complex and defects in nucleation of sub\u2010pellicular microtubules, polarization and symmetrical assembly of daughter parasites during repeated endodyogeny. TgVps45 interacts with the SNARE protein TgStx16 and TgVAMP4\u20101. Conditional ablation of TgStx16 causes the similar growth defect like TgVps45 deficiency suggested they work together for the vesicle fusion at TGN. These findings indicate that these two SM proteins are crucial for assembly of pellicle and sub\u2010pellicle network in\n                    <jats:italic>T. gondii<\/jats:italic>\n                    respectively.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14411","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T09:41:05Z","timestamp":1572514865000},"page":"208-221","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Sec1\/Munc18\u2010like proteins TgSec1 and TgVps45 play pivotal roles in assembly of the pellicle and sub\u2010pellicle network in\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"113","author":[{"given":"Shinuo","family":"Cao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin People\u2019s Republic of 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