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Here, we show that mutations in the energy transducer TonB, encoded by\n                    <jats:italic>smlt0009<\/jats:italic>\n                    in\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    , confer ceftazidime resistance and\n                    <jats:italic>smlt0009<\/jats:italic>\n                    mutants have reduced uptake of ceftazidime. This breaks the dogma that \u03b2\u2010lactams enter Gram\u2010negative bacteria only by passive diffusion through outer membrane porins. We also show that ceftazidime\u2010resistant TonB mutants are cross\u2010resistant to fluoroquinolone antimicrobials and a siderophore\u2010conjugated lactivicin antibiotic designed to target TonB\u2010dependent uptake. This implies that attempts to improve the penetration of antimicrobials into\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    by conjugating them with TonB substrates will suffer from the fact that \u03b2\u2010lactams and fluoroquinolones coselect resistance to these novel and otherwise promising antimicrobials. Finally, we show that\n                    <jats:italic>smlt0009<\/jats:italic>\n                    mutants already exist among\n                    <jats:italic>S. maltophilia<\/jats:italic>\n                    clinical isolates and have reduced susceptibility to siderophore\u2010conjugated lactivicin, despite the in vitro growth impairment seen in\n                    <jats:italic>smlt0009<\/jats:italic>\n                    mutants selected in the laboratory.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14434","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T03:03:55Z","timestamp":1574823835000},"page":"492-503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["TonB\u2010dependent uptake of \u03b2\u2010lactam antibiotics in the opportunistic human pathogen\n                    <i>Stenotrophomonas maltophilia<\/i>"],"prefix":"10.1111","volume":"113","author":[{"given":"Karina","family":"Calvopi\u00f1a","sequence":"first","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine University of Bristol Bristol United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Punyawee","family":"Dulyayangkul","sequence":"additional","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine University of Bristol Bristol United Kingdom"},{"name":"Program in Applied Biological Sciences Environmental Health, Chulabhorn Graduate Institute Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate J.","family":"Heesom","sequence":"additional","affiliation":[{"name":"University of Bristol Proteomics Facility Bristol United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1454-8440","authenticated-orcid":false,"given":"Matthew B.","family":"Avison","sequence":"additional","affiliation":[{"name":"School of Cellular &amp; Molecular Medicine University of Bristol Bristol United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,11]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Performance standards for antimicrobial disk suceptibility tests; Approved standard","author":"Clinical and Laboratory Standards Institute","year":"2006"},{"key":"e_1_2_7_3_1","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically","author":"Clinical and Laboratory Standards Institute","year":"2012"},{"key":"e_1_2_7_4_1","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"Clinical and Laboratory Standards Institute","year":"2017"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2144\/99265bm05"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/49.2.387"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/46.6.879"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00019-11"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00638-18"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13831"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00371-16"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.03681-14"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01558-19"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/13543784.2018.1426745"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq169"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-4-r74"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.84"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03515.x"},{"key":"e_1_2_7_21_1","first-page":"150","article-title":"Siderophores of Stenotrophomonas maltophilia: Detection and determination of their chemical nature","volume":"44","author":"Garcia C. 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