{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:35:25Z","timestamp":1790724925985,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T00:00:00Z","timestamp":1579737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI119437"],"award-info":[{"award-number":["AI119437"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI146909"],"award-info":[{"award-number":["AI146909"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Borrelia burgdorferi<\/jats:italic> encodes a functional homolog of canonical Lon protease termed Lon\u20102. To date, the contribution of Lon\u20102 to <jats:italic>B. burgdorferi<\/jats:italic> fitness and infection remains unexplored. Herein, we showed that expression of <jats:italic>lon\u20102<\/jats:italic> was highly induced during animal infection, suggesting that Lon\u20102 is important for <jats:italic>B. burgdorferi<\/jats:italic> infection. We further generated a <jats:italic>lon\u20102<\/jats:italic> deletion mutant. Compared with that of wild\u2010type (WT) strain, the infectivity of the mutant was severely attenuated in a murine infection model. Although no growth defect was observed for the mutant in normal BSK\u2010II medium, resistance of the <jats:italic>lon\u20102<\/jats:italic> mutant to osmotic stress was markedly reduced. In addition, when exposed to <jats:italic>tert<\/jats:italic>\u2010Butyl hydroperoxide, survival of the <jats:italic>lon\u20102<\/jats:italic> mutant was impaired. In addition, we found that the protein levels of RpoS and RpoS\u2010dependent OspC were decreased in the mutant. All these phenotypes were restored to WT or near\u2010WT levels when <jats:italic>lon\u20102<\/jats:italic> mutation was complemented in <jats:italic>cis<\/jats:italic>. Taken together, these results demonstrate that Lon\u20102 is critical for <jats:italic>B. burgdorferi<\/jats:italic> to establish infection and to cope with environmental stresses. This study provides a foundation for further uncovering the direct link between the dual roles of Lon\u20102 in protein quality control and bacterial pathogenesis.<\/jats:p>","DOI":"10.1111\/mmi.14460","type":"journal-article","created":{"date-parts":[[2020,1,19]],"date-time":"2020-01-19T06:39:07Z","timestamp":1579415947000},"page":"938-950","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["The Lon\u20102 protease of <i>Borrelia burgdorferi<\/i> is critical for infection in the mammalian host"],"prefix":"10.1111","volume":"113","author":[{"given":"Charlotte","family":"Mason","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Thompson","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7427-1856","authenticated-orcid":false,"given":"Zhiming","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.133"},{"key":"e_1_2_9_3_1","first-page":"959","article-title":"Chronic Lyme borreliosis in the laboratory mouse","volume":"143","author":"Barthold S. 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