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Furthermore, the cyanobacterial divisome includes proteins specific for both the Gram types as well as cyanobacteria\u2010specific proteins. Cells in multicellular cyanobacteria share a continuous periplasm and their cytoplasms are connected by septal junctions that enable communication between cells in the filament. The localization of septal junction proteins depends on interaction with the divisome, however additional yet unknown proteins may be involved in this process. Here, we characterized Alr3364 (termed SepI), a novel septal protein that interacts with the divisome in the multicellular heterocystous cyanobacterium <jats:italic>Anabaena<\/jats:italic> sp. strain PCC 7120. SepI localized to the Z\u2010ring and the intercellular septa but did not interact with FtsZ. Instead, SepI interacted with the divisome proteins ZipN, SepF and FtsI and with the septal protein SepJ. The inactivation of <jats:italic>sepI<\/jats:italic> led to a defect in cell filament integrity, colony and cell morphology, septum size, nanopore formation and peptidoglycan biogenesis, and inability to differentiate heterocysts. Our results show that SepI plays a role in intercellular communication and furthermore indicate that SepI functions in the coordination of septal junction localization during cell division.<\/jats:p>","DOI":"10.1111\/mmi.14483","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T12:29:32Z","timestamp":1581337772000},"page":"1140-1154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["A novel septal protein of multicellular heterocystous cyanobacteria is associated with the divisome"],"prefix":"10.1111","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3461-5391","authenticated-orcid":false,"given":"Benjamin L.","family":"Springstein","sequence":"first","affiliation":[{"name":"Institute of General Microbiology Christian\u2010Albrechts\u2010Universit\u00e4t zu Kiel Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ar\u00e9valo","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis CSIC and Universidad de Sevilla Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas O.","family":"Helbig","sequence":"additional","affiliation":[{"name":"AG Proteomics &amp; Bioanalytics Institute for Experimental Medicine Christian\u2010Albrechts\u2010Universit\u00e4t zu Kiel Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1071-6590","authenticated-orcid":false,"given":"Antonia","family":"Herrero","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis CSIC and Universidad de Sevilla Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2783-4342","authenticated-orcid":false,"given":"Karina","family":"Stucken","sequence":"additional","affiliation":[{"name":"Department of Food Engineering Universidad de La Serena La Serena Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7605-7343","authenticated-orcid":false,"given":"Enrique","family":"Flores","sequence":"additional","affiliation":[{"name":"Instituto de Bioqu\u00edmica Vegetal y Fotos\u00edntesis CSIC and Universidad de Sevilla Seville Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9042-192X","authenticated-orcid":false,"given":"Tal","family":"Dagan","sequence":"additional","affiliation":[{"name":"Institute of General Microbiology Christian\u2010Albrechts\u2010Universit\u00e4t zu Kiel Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00386"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13929"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz297"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02128-14"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39336-6"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3178"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02260"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00421-5"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.01.007"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90605-1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.11.011"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/life9010001"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00085-07"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpy.12934"},{"key":"e_1_2_9_17_1","volume-title":"The cell envelope. 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