{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:05:09Z","timestamp":1790435109074,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,2,18]],"date-time":"2021-02-18T00:00:00Z","timestamp":1613606400000},"content-version":"am","delay-in-days":338,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI046223"],"award-info":[{"award-number":["R01AI046223"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI\u20101627442"],"award-info":[{"award-number":["DBI\u20101627442"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    is an opportunistic pathogen in humans, responsible for approximately 20% of disseminated candidiasis.\n                    <jats:italic>Candida glabrata's<\/jats:italic>\n                    ability to adhere to host tissue is mediated by GPI\u2010anchored cell wall proteins (GPI\u2010CWPs); the corresponding genes contain long tandem repeat regions. These repeat regions resulted in assembly errors in the reference genome. Here, we performed a de novo assembly of the\n                    <jats:italic>C. glabrata<\/jats:italic>\n                    type strain CBS138 using long single\u2010molecule real\u2010time reads, with short read sequences (Illumina) for refinement, and constructed telomere\u2010to\u2010telomere assemblies of all 13 chromosomes. Our assembly has excellent agreement overall with the current reference genome, but we made substantial corrections within tandem repeat regions. Specifically, we removed 62 genes of which 45 were scrambled due to misassembly in the reference. We annotated 31 novel ORFs of which 24 ORFs are GPI\u2010CWPs. In addition, we corrected the tandem repeat structure of an additional 21 genes. Our corrections to the genome were substantial, with the length of new genes and tandem repeat corrections amounting to approximately 3.8% of the ORFeome length. As most corrections were within the coding regions of GPI\u2010CWP genes, our genome assembly establishes a high\u2010quality reference set of genes and repeat structures for the functional analysis of these cell surface proteins.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14488","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T06:48:35Z","timestamp":1582008515000},"page":"1209-1224","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["De novo genome assembly of\n                    <i>Candida glabrata<\/i>\n                    reveals cell wall protein complement and structure of dispersed tandem repeat arrays"],"prefix":"10.1111","volume":"113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4254-8379","authenticated-orcid":false,"given":"Zhuwei","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics Johns Hopkins 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