{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:21:56Z","timestamp":1767626516556,"version":"3.40.5"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T00:00:00Z","timestamp":1583712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102015\/2020\u201004878"],"award-info":[{"award-number":["RGPIN\u20102015\/2020\u201004878"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The methionine salvage pathway (MSP) regenerates methionine from 5\u2032\u2010methylthioadenosine (MTA). Aerobic MSP consists of six enzymatic steps. The <jats:italic>mug14<jats:sup>+<\/jats:sup><\/jats:italic> and <jats:italic>adi1<jats:sup>+<\/jats:sup><\/jats:italic> genes that are involved in the third and fifth steps of the pathway are repressed when <jats:italic>Schizosaccharomyces pombe<\/jats:italic> undergoes a transition from high\u2010 to low\u2010iron conditions. Results consistently show that methionine auxotrophic cells (<jats:italic>met6\u0394<\/jats:italic>) require iron for growth in the presence of MTA as the sole source of methionine. Inactivation of the iron\u2010using protein Adi1 leads to defects in the utilization of MTA. In the case of the third step of the pathway, co\u2010expression of two distinct proteins, Mta3 and Mde1, is required. These proteins are interdependent to rescue MTA\u2010dependent growth deficit of <jats:italic>met6\u0394<\/jats:italic> cells. Coimmunoprecipitation experiments showed that Mta3 is a binding partner of Mde1. Meiotic <jats:italic>met6\u0394<\/jats:italic> cells co\u2010expressing <jats:italic>mta3<jats:sup>+<\/jats:sup><\/jats:italic> and <jats:italic>mde1<jats:sup>+<\/jats:sup><\/jats:italic> or <jats:italic>mta3<jats:sup>+<\/jats:sup><\/jats:italic> and <jats:italic>mug14<jats:sup>+<\/jats:sup><\/jats:italic> produce comparable levels of spores in the presence of MTA, revealing that Mde1 and Mug14 share a common function when co\u2010expressed with Mta3 in sporulating cells. In sum, our findings unveil several novel features of MSP, especially with respect to its regulation by iron and the discovery of a non\u2010canonical third enzymatic step in the fission yeast.<\/jats:p>","DOI":"10.1111\/mmi.14495","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T07:54:29Z","timestamp":1582530869000},"page":"46-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Iron deficiency leads to repression of a non\u2010canonical methionine salvage pathway in <i>Schizosaccharomyces pombe<\/i>"],"prefix":"10.1111","volume":"114","author":[{"given":"Ariane","family":"Brault","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biochimie et de G\u00e9nomique Fonctionnelle Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9 Universit\u00e9 de Sherbrooke Sherbrooke QC 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