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One important virulence factor is its urease enzyme, which requires nickel to be catalytically active. It is, therefore, hypothesized that nickel import is critical for <jats:italic>P. mirabilis<\/jats:italic> urease activity and pathogenesis during infection. <jats:italic>P. mirabilis<\/jats:italic> strain HI4320 encodes two putative nickel import systems, designated Nik and Ynt. By disrupting the substrate\u2010binding proteins from each import system (<jats:italic>nikA<\/jats:italic> and <jats:italic>yntA<\/jats:italic>), we show that Ynt is the primary nickel importer, while Nik only compensates for loss of Ynt at high nickel concentrations. We further demonstrate that these are the only binding proteins capable of importing nickel for incorporation into the urease enzyme. Loss of either nickel\u2010binding protein results in a significant fitness defect in a murine model of CAUTI, but YntA is more crucial as the <jats:italic>yntA<\/jats:italic> mutant was significantly outcompeted by the <jats:italic>nikA<\/jats:italic> mutant. Furthermore, despite the importance of nickel transport for hydrogenase activity, the sole contribution of <jats:italic>yntA<\/jats:italic> and <jats:italic>nikA<\/jats:italic> to virulence is due to their role in urease activity, as neither mutant exhibited a fitness defect when disrupted in a urease\u2010negative background.<\/jats:p>","DOI":"10.1111\/mmi.14505","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T10:03:24Z","timestamp":1586253804000},"page":"185-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Ynt is the primary nickel import system used by <i>Proteus mirabilis<\/i> and specifically contributes to fitness by supplying nickel for urease activity"],"prefix":"10.1111","volume":"114","author":[{"given":"Aimee L.","family":"Brauer","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Jacobs School of Medicine and Biomedical Sciences State University of New York at Buffalo Buffalo NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian S.","family":"Learman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Jacobs School of Medicine and Biomedical Sciences State University of New York at Buffalo Buffalo NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2165-2759","authenticated-orcid":false,"given":"Chelsie E.","family":"Armbruster","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Jacobs School of Medicine and Biomedical Sciences State University of New York at Buffalo Buffalo NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.330365"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0009-2017"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006434"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007653"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.02136-12"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00808-16"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit663"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.7.2877-2880.1996"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2013.09.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1220341110"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R112.446526"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)50311-0"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2015.153"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000471707"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)41592-8"},{"key":"e_1_2_9_17_1","first-page":"346","article-title":"Urease. 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