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To maintain a functional OM, cells require distinct mechanisms to ensure balance of proteins and lipids in the membrane. Mutations in OM biogenesis and\/or homeostasis pathways often result in permeability defects, but how molecular changes in the OM affect barrier function is unclear. Here, we seek potential mechanism(s) that can alleviate permeability defects in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    cells lacking the Tol\u2010Pal complex, which accumulate excess PLs in the OM. We identify mutations in enterobacterial common antigen (ECA) biosynthesis that re\u2010establish OM barrier function against large hydrophilic molecules, yet did not restore lipid homeostasis. Furthermore, we demonstrate that build\u2010up of biosynthetic intermediates, but not loss of ECA itself, contributes to the rescue. This suppression of OM phenotypes is unrelated to known effects that accumulation of ECA intermediates have on the cell wall. Finally, we reveal that an unusual diacylglycerol pyrophosphoryl\u2010linked lipid species also accumulates in ECA mutants, and might play a role in the rescue phenotype. Our work provides insights into how OM barrier function can be restored independent of lipid homeostasis, and highlights previously unappreciated effects of ECA\u2010related species in OM biology.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14590","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T15:55:10Z","timestamp":1598543710000},"page":"991-1005","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mutations in enterobacterial common antigen biosynthesis restore outer membrane barrier function in\n                    <i>Escherichia coli tol\u2010pal<\/i>\n                    mutants"],"prefix":"10.1111","volume":"114","author":[{"given":"Xiang\u2019Er","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Chemistry National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wee Boon","family":"Tan","sequence":"additional","affiliation":[{"name":"Singapore Center for Environmental Life Sciences Engineering National University of Singapore (SCELSE\u2010NUS) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Shrivastava","sequence":"additional","affiliation":[{"name":"Department of Chemistry National University of Singapore Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah Chwee San","family":"Seow","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swaine Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore"},{"name":"Genome Institute of Singapore, Agency for Science, Technology and Research (A*STAR) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue Li","family":"Guan","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine Nanyang Technological University Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5466-7183","authenticated-orcid":false,"given":"Shu\u2010Sin","family":"Chng","sequence":"additional","affiliation":[{"name":"Department of Chemistry National University of Singapore Singapore Singapore"},{"name":"Singapore Center for Environmental Life Sciences Engineering National University of Singapore (SCELSE\u2010NUS) Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"issue":"1","key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/jb.168.1.348-356.1986","article-title":"Immunocytochemical localization of enterobacterial 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