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In <jats:italic>Lp<\/jats:italic>, the sigma factor RpoS is essential for survival in water. A previous transcriptomic study showed that RpoS positively regulates the small regulatory RNA Lpr10. In the present study, deletion of <jats:italic>lpr10<\/jats:italic> results in an increased survival of <jats:italic>Lp<\/jats:italic> in water. Microarray analysis and RT\u2010qPCR revealed that Lpr10 negatively regulates the expression of RpoS in the postexponential phase. Electrophoretic mobility shift assay and in\u2010line probing showed that Lpr10 binds to a region upstream of the previously identified transcription start sites (TSS) of <jats:italic>rpoS<\/jats:italic>. A third putative transcription start site was identified by primer extension analysis, upstream of the Lpr10 binding site. In addition, <jats:italic>nlpD<\/jats:italic> TSS produces a polycistronic mRNA including the downstream gene <jats:italic>rpoS<\/jats:italic>, indicating a fourth TSS for <jats:italic>rpoS<\/jats:italic>. Our results suggest that the transcripts from the third and fourth TSS are negatively regulated by the Lpr10 sRNA. Therefore, we propose that Lpr10 is involved in a negative regulatory feedback loop to maintain expression of RpoS to an optimal level.<\/jats:p>","DOI":"10.1111\/mmi.14644","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T04:16:08Z","timestamp":1605500168000},"page":"789-806","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The small regulatory RNA Lpr10 regulates the expression of RpoS in <i>Legionella pneumophila<\/i>"],"prefix":"10.1111","volume":"115","author":[{"given":"Joseph","family":"Saoud","sequence":"first","affiliation":[{"name":"Department of Natural Resource Sciences McGill University Sainte\u2010Anne\u2010de\u2010Bellevue QC Canada"},{"name":"Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA) Universit\u00e9 de Montr\u00e9al, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Saint\u2010Hyacinthe QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Claude","family":"Carrier","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Functional Genomics, RNA Group Universit\u00e9 de Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5253-1415","authenticated-orcid":false,"given":"\u00c9ric","family":"Mass\u00e9","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Functional Genomics, RNA Group Universit\u00e9 de Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2721-7715","authenticated-orcid":false,"given":"S\u00e9bastien P.","family":"Faucher","sequence":"additional","affiliation":[{"name":"Department of Natural Resource Sciences McGill University Sainte\u2010Anne\u2010de\u2010Bellevue QC Canada"},{"name":"Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA) Universit\u00e9 de Montr\u00e9al, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Saint\u2010Hyacinthe QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68491-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep33998"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01493-07"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20190173"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294-016-0663-6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02465.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2468-2476.2004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102946"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3029-3034.1998"},{"key":"e_1_2_8_12_1","first-page":"1083","article-title":"Legionellosis \u2010 United States, 2000\u20102009","volume":"60","author":"Centers for Disease and Prevention","year":"2011","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094226"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000117"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2020.194506"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.182493.111"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00882-09"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1909374116"},{"volume-title":"ECDC. 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