{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:43:41Z","timestamp":1792806221469,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T00:00:00Z","timestamp":1637539200000},"content-version":"am","delay-in-days":344,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP110103144"],"award-info":[{"award-number":["DP110103144"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1182369"],"award-info":[{"award-number":["GNT1182369"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Iron\u2013sulfur (Fe\u2010S) clusters are prosthetic groups on proteins that function in a range of enzymatic and electron transfer reactions. Fe\u2010S cluster synthesis is essential for the survival of all eukaryotes. Independent Fe\u2010S cluster biosynthesis pathways occur in the mitochondrion, plastid, and cytosolic compartments of eukaryotic cells. Little is known about the cytosolic Fe\u2010S cluster biosynthesis in apicomplexan parasites, the causative agents of diseases such as malaria and toxoplasmosis. NBP35 serves as a key scaffold protein on which cytosolic Fe\u2010S clusters assemble, and has a cytosolic localization in most eukaryotes studied thus far. Unexpectedly, we found that the NBP35 homolog of the apicomplexan\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    (\n                    <jats:italic>Tg<\/jats:italic>\n                    NBP35) localizes to the outer mitochondrial membrane, with mitochondrial targeting mediated by an N\u2010terminal transmembrane domain. We demonstrate that\n                    <jats:italic>Tg<\/jats:italic>\n                    NBP35 is critical for parasite proliferation, but that, despite its mitochondrial localization, it is not required for Fe\u2010S cluster synthesis in the mitochondrion. Instead, we establish that\n                    <jats:italic>Tg<\/jats:italic>\n                    NBP35 is important for the biogenesis of cytosolic Fe\u2010S proteins. Our data are consistent with\n                    <jats:italic>Tg<\/jats:italic>\n                    NBP35 playing a central and specific role in cytosolic Fe\u2010S cluster biosynthesis, and imply that the assembly of cytosolic Fe\u2010S clusters occurs on the cytosolic face of the outer mitochondrial membrane in these parasites.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14651","type":"journal-article","created":{"date-parts":[[2020,11,22]],"date-time":"2020-11-22T06:19:24Z","timestamp":1606025964000},"page":"968-985","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A key cytosolic iron\u2013sulfur cluster synthesis protein localizes to the mitochondrion of\n                    <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"115","author":[{"given":"Yi Tong Vincent","family":"Aw","sequence":"first","affiliation":[{"name":"Research School of Biology Australian National University Canberra ACT Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azadeh","family":"Seidi","sequence":"additional","affiliation":[{"name":"Research School of Biology Australian National University Canberra ACT Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8228-8797","authenticated-orcid":false,"given":"Jenni A.","family":"Hayward","sequence":"additional","affiliation":[{"name":"Research School of Biology Australian National University Canberra ACT Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Centre for Advanced Microscopy Australian National University Canberra ACT Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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