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The outer membrane porin OmpU confers bacterial survival and colonization advantages in the presence of host\u2010derived antimicrobial peptides as well as bile. Expression of\n                    <jats:italic>ompU<\/jats:italic>\n                    is controlled by the virulence regulator ToxR.\n                    <jats:italic>rpoE<\/jats:italic>\n                    knockouts are accompanied by suppressor mutations causing\n                    <jats:italic>ompU<\/jats:italic>\n                    downregulation. Therefore, OmpU constitutes an intersection of the ToxR regulon and the \u03c3\n                    <jats:sup>E<\/jats:sup>\n                    \u2010pathway in\n                    <jats:italic>V<\/jats:italic>\n                    .\n                    <jats:italic>cholerae<\/jats:italic>\n                    . To understand the mechanism by which the sigma factor \u03c3\n                    <jats:sup>E<\/jats:sup>\n                    regulates OmpU synthesis, we performed transcription studies using\n                    <jats:italic>ompU<\/jats:italic>\n                    reporter fusions and immunoblot analysis. Our data revealed an increase in\n                    <jats:italic>ompU<\/jats:italic>\n                    promoter activity in \u0394\n                    <jats:italic>rpoE<\/jats:italic>\n                    strains, as well as in a \u0394\n                    <jats:italic>ompU<\/jats:italic>\n                    background, indicating a negative feedback regulation circuit of\n                    <jats:italic>ompU<\/jats:italic>\n                    expression. This regulation seems necessary, since elevated lethality rates of \u0394\n                    <jats:italic>rpoE<\/jats:italic>\n                    strains occur upon\n                    <jats:italic>ompU<\/jats:italic>\n                    overexpression. Manipulation of OmpU\u2019s C\u2010terminal portion revealed its relevance for protein stability and potency of \u03c3\n                    <jats:sup>E<\/jats:sup>\n                    release. Furthermore, \u0394\n                    <jats:italic>rpoE<\/jats:italic>\n                    strains are still capable of elevating OmpU levels under membrane stress conditions triggered by the bile salt sodium deoxycholate. This study provides new details about the impact of \u03c3\n                    <jats:sup>E<\/jats:sup>\n                    on\n                    <jats:italic>ompU<\/jats:italic>\n                    regulation, which is critical to the pathogen\u2019s intestinal survival.\n                  <\/jats:p>","DOI":"10.1111\/mmi.14669","type":"journal-article","created":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T22:50:29Z","timestamp":1608245429000},"page":"1244-1261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["\u03c3\n                    <sup>E<\/sup>\n                    controlled regulation of porin OmpU in\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1111","volume":"115","author":[{"given":"Nina","family":"Pennetzdorfer","sequence":"first","affiliation":[{"name":"Institute of Molecular Biosciences University of Graz Graz 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